Influence of Instaflex Advanced Supplement on Joint Function

The research procedures will be conducted at the Human Performance Laboratory (Room 1201, Plants for Human Health Institute Building, 600 Laureate Way), operated by Appalachian State University at the North Carolina Research Campus (NCRC) in Kannapolis, NC. Subjects will come here for orientation/baseline testing and then 3 other lab visits (each session will take about 30 minutes). The total amount of time study participants will be asked to volunteer for this study is about 2 hours at the Human Performance Laboratory (over a 12-week period). Participants will also take a daily supplement for 12 weeks and keep compliant with this regimen.

Lab visit testing sequence:

• First visit:

• Consent form
• Medical health questionnaire to verify medical history, and lifestyle habits.
• Questionnaire for previous 4-weeks symptoms.
• Height, weight, and percent body fat (BIA).Testing will take place behind a screened area of the lab to maintain privacy.
• Questionnaires: WOMAC, visual pain scale, POMS.
• Receive supplement organizer tray with 4-weeks supply (and instructions).
• After a practice trial, study participants will walk as quickly and far as they can on a track for 6 minutes. Total distance covered in 6 minutes will be recorded.
• 4 weeks, 8-weeks, 12 weeks (second, third, and fourth lab visits):

• Symptoms log for previous 4-weeks symptomatology.
• Height, weight, and percent body fat.
• Questionnaires: WOMAC, visual pain scale, POMS.
• Receive supplement organizer tray with 4-weeks supply (and instructions) during second and third lab visits.
• 6-minute walk test

Supplements:

Supplements (Instaflex Advanced, placebo) will be contained in identical looking capsules and organized by day of the week in supplement organizer trays. Study participants will take 1 capsule each day in the morning. The placebo capsules contain magnesium stearate, an inert substance. The Instaflex Advanced supplement ingredients include: turmeric, resveratrol, Boswellia serrata, collagen, hyaluronic acid, and black pepper extract. Compliance will be monitored with bi-weekly email messages, and by counting unused capsules when the supplement trays every 4 weeks. Acetaminophen, as found in Tylenol, will be allowed as a rescue medicine for pain during the study as needed.

Inclusion criteria

• Male or female adult
• Age 50-75 years
• Self-reported history (>3 months) of joint discomfort in the knees. NOTE: the severity of knee discomfort will be determined through a questionnaire called the WOMAC, and should be at least 2 points (indicating mild knee discomfort or greater).
• No history of regular use of non-steroidal anti-inflammatory drugs (NSAIDs) use (e.g., ibuprofen, aspirin) during the previous two weeks, and willingness to avoid the use of NSAIDs during the 12-week study.
• Not on other medications (e.g., analgesic gels, arthritis medications, other anti-inflammatory drugs) or supplements (in particular, glucosamine and chondroitin) for joint discomfort during the previous two weeks, and a willingness to avoid use of these during the 12-week study.
• No serious medical problems (current cancer case, severe rheumatoid arthritis, recent heart attack, recent stroke, congestive heart failure, ulcers, kidney disease, or other disease that would interfere with study participation).
• No psychiatric disorder or other condition that might interfere with self-assessment ability.
• Willingness to follow all study procedures including randomization to one of two groups, and to stay weight stable during the study.

Exclusion criteria

• Inability to comply with study requirements including avoidance of NSAIDs, other anti-inflammatory medications, or discomfort supplements.
• Current diagnosis of cardiovascular disease, cancer (except for non-melanoma skin cancer), severe rheumatoid arthritis, ulcers, kidney disease, psychiatric disorder.
• For female subjects: Pregnant or nursing, or planning to be pregnant or nursing during the study.
• On a weight loss program during the study.
• Vegetarian or vegan.