University of Reading
Reading, RG6 6DZ, United Kingdom
NCT Number: NCT05581615
The aim of the study is to investigate whether the food matrix has an influence on the prebiotic efficacy (bifidobacteria growth) of chicory-derived Orafti® inulin in healthy adult volunteers. The main question it aims to answer is:
Does the food matrix influence the prebiotic effect of chicory-derived inulin in terms of stimulation of bifidobacteria in the gut microbiota Participants will receive one of three food items containing inulin or pure inulin dissolved in water daily for 10 days. The food items or pure inulin powder will deliver 10 g/d inulin split into two dosages of 5 g/d. They will provide stool samples before and at the end of the intake period. They will record any gastrointestinal sensations and bowel habits in a diary. Urinary samples will be collected at baseline and end of intervention for analysis of metabolites with Nuclear Magnetic resonance (NMR).
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Notify Me18 year–65 year
All sexes
Interventional
Not applicable
Reading, RG6 6DZ, United Kingdom
Healthy volunteers accepted: Yes
Only the study team can determine whether someone qualifies for participation.
Inclusion criteria
Exclusion criteria
Each intervention provided 10 g/d inulin split into two dosages of 5 g.
Time frame: 10 days
Bifidobacterium spp. will be assessed by fluorescence in situ hybridisation - flow cytometry (FISH-FLOW) and 16S rRNA gene sequencing
Time frame: 10 days
Total bacteria will be assessed by fluorescence in situ hybridisation - flow cytometry (FISH-FLOW)
Time frame: 10 days
Bacterial numbers will be assessed by fluorescence in situ hybridisation - flow cytometry (FISH-FLOW) and 16S rRNA gene sequencing
Time frame: 10 days
Numbers of stools will be recorded in daily diary
Time frame: 10 days
Stool consistency will be recorded in daily diary according to Bristol Stool Form Scale
Time frame: 10 days
Bloating will be recorded in daily diary according to a 4-point scale
Time frame: 10 days
Abdominal pressure will be recorded in daily diary according to a 4-point scale
Time frame: 10 days
Abdominal pain will be recorded in daily diary according to a 4-point scale
Time frame: 10 days
Flatulence will be recorded in daily diary according to a 4-point scale
Time frame: 10 days
Urinary metabolites will be analysed by Nuclear magnetic resonance (NMR)
Beneo-Institute
Industry
Randomized Four-arm Trial Investigating the Effects of Different Food Matrices on the Prebiotic Efficacy of Inulin Type Fructans (ITF) From Chicory Root
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