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Completed

NCT Number: NCT05131555

Influence of Endurance Exercise and Histamine Receptors on the Gene Expression in Skeletal Muscle

Blocking histamine H1/H2 receptors blunts chronic endurance training adaptations. The current study addresses a twofold research question: "What is the influence of endurance training (1) and histamine H1 and H2 signaling (2) on the gene expression in human skeletal muscle." Results from this study will yield more insights into the molecular mechanisms of adaptations to exercise training.

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Key information

Conditions

Exercise Behavior Histamine Motor Activity

Age range

18 year–45 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Department of movement and sports sciences, Ghent University, Belgium

Ghent, Oost-Vlaanderen, 9000, Belgium

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Male and female
  • 18-45 years
  • not to medium physically active

Exclusion criteria

  • Smoking
  • Chronic disease
  • Supplement or medication intake
  • Seasonal allergies

Treatment and study plan

Placebo: Placebo + exercise training.

Drug

Oral placebo Exercise training: Interval-based cycling exercise

H1 blockade: H1 receptor antagonist + exercise training

Drug

H1 blockade: oral blockade Exercise training: Interval-based cycling exercise

H2 Blockade: H2 receptor antagonist + exercise training

Drug

H2 blockade: oral blockade Exercise training: Interval-based cycling exercise

Primary outcomes

  1. Change in muscle transcriptome (direct response)

    Time frame: On each test-day: 0 minutes after the exercise training versus at rest.

    RNA-sequencing

  2. Change in muscle transcriptome (delayed response)

    Time frame: On each test-day: 180 minutes after the exercise training versus at rest.

    RNA-sequencing

  3. Muscle glycogen depletion

    Time frame: On each test-day: 0 minutes after the exercise training versus at rest.

    Fluorometric determination of muscle glycogen levels

  4. Muscle glycogen resynthesis

    Time frame: On each test-day: 180 minutes after the exercise training versus at rest.

    Fluorometric determination of muscle glycogen levels

  5. Plasma volume change

    Time frame: Change from rest to different time-points after exercise (0, 30, 60, 120 and 180 minutes after the exercise training).

    Plasma volume based on hemoglobin and hematocrit concentration.

Secondary outcomes

  1. Heart rate during the exercise training

    Time frame: Continuously during training on each test-day.

    Heart rate during the exercise training.

  2. Blood lactate

    Time frame: On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.

    Capillary lactate concentration.

  3. Blood glucose

    Time frame: On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.

    Capillary glucose concentration.

  4. Blood histamine

    Time frame: On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.

    histamine concentration in blood samples.

  5. Blood insulin

    Time frame: On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.

    insulin concentration in blood samples.

  6. Muscle signaling pathways relevant for glucose metabolism and cardiovascular health

    Time frame: On each test-day: at rest, 0 minutes and 180 minutes after the exercise training.

    Phosphorylation status of proteins assessed by Western Blotting

Sponsors and collaborators

Lead sponsor

University Ghent

Other

Collaborators

  • Research Foundation Flanders

Registry information

Official study title

Influence of Endurance Training and Histamine Receptor Antagonists on the Transcriptome Response in Human Muscle

Important dates

Study start
2021
Primary completion
2021
Study completion
2021
First posted
Nov 23, 2021
Registry last updated
Apr 12, 2024

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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