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Completed

NCT Number: NCT06890741

Inflamation, Gut Leakage, Iron Status After 6000m Ergometer Test in Elite Rowers.

The influence of a 6000m ergometer test on the markers of gut injury, endotoxemia, iron status, stress and inflammation in elite rowers.

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Key information

Conditions

Endothenial Dysfunction Iron Status Oxidative Stress

Age range

18 year–24 year

Sex eligibility

All sexes

Study type

Observational

Primary location

Poznan University of Physical Education

Gorzów Wielkopolski, Lubusz Voivodeship, 66-400, Poland

About this study

The principal objective of our study was to assess gut permeability after completing a 6000-meter rowing test. The study was performed at the beginning of a competitive training phase. Eighteen elite Polish Rowing Team rowers participated in the study after applying the inclusion/exclusion criteria. The participants performed a 6000-meter ergometer test. Blood samples were taken before the test, after exercise, and after 1 hour of restitution. Parameters, such as I-FABP (intestinal fatty acid binding protein), LPS (lipopolysaccharide), LBP (lipopolysaccharide binding protein), claudin3, ferritin, transferrin, hepcidin, IL-6 (Interleukin-6), HO-1 (Heme Oxygenase 1), and ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) were determined using appropriate biochemical tests.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • minimum five years of training,
  • five training sessions per week minimum,
  • total training time minimum of 240 minutes,
  • membership in the Polish Rowing Team,
  • finishing the 6000-meter ergometer test.

Exclusion criteria

  • antibiotic therapy within the last three months,
  • probiotics and prebiotics within the last three months,
  • dietary regime,
  • gastrointestinal diseases.

Treatment and study plan

Exercise test

Other

Participant perforemed 6000m ergometer test.

Primary outcomes

  1. I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury

    Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period

    I-FABP concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays

  2. LBP (lipopolysaccharide binding protein) to measure endotoxin

    Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period

    LBP concentration measured in blood [ug/ml] using commercially available enzyme-linked immunosorbent assays

  3. Claudin 3 to measure tight junction leakage

    Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period

    claudin 3 concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays

  4. LPS (lipopolysaccharide) to measure endotoxin

    Time frame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period

    LPS concentration measured in blood [EU/Ll] using commercially available enzyme-linked immunosorbent assays

  5. Lactic acid to measure fatigue after the race

    Time frame: Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test.

    Lactate concentration measured in capillary blood from earlobe [mmol/L] using a commercially available kit (Diaglobal, Berlin, Germany)

  6. Ferritin to measure iron status

    Time frame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.

    Ferritin concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays

  7. Transferrin to measure iron status

    Time frame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.

    Transferrin concentration measured in blood [mg/ml] using commercially available enzyme-linked immunosorbent assays

  8. Hepcidin to measure iron status

    Time frame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.

    Hepcidin concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays

  9. IL-6 (Interleukin-6) to measure inflammation

    Time frame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.

    IL-6 concentration measured in blood [ng/l] using commercially available enzyme-linked immunosorbent assays.

  10. HO-1 (Heme Oxygenase 1) to measure oxidative stress

    Time frame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.

    HO-1 concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays

  11. ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) to measure oxidative stress

    Time frame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.

    ACSL4 concentration measured in blood [pg/ml] using commercially available enzyme-linked immunosorbent assays.

Secondary outcomes

  1. energy

    Time frame: day before test, in the morning before the test

    energy [kcal ]of food intake measured by 24h dietary recall

  2. protein

    Time frame: day before test, in the morning before the test

    protein [g ]of food intake measured by 24h dietary recall

  3. carbohydrate

    Time frame: day before test, in the morning before the test

    carbohydrate [g ]of food intake measured by 24h dietary recall

  4. fat

    Time frame: day before test, in the morning before the test

    fat [g ]of food intake measured by 24h dietary recall

  5. fiber

    Time frame: day before test, in the morning before the test

    fiber [g ]of food intake measured by 24h dietary recall

  6. body mass

    Time frame: in the morning before the test

    Body mass [kg] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).

  7. Body composition - water

    Time frame: in the morning before the test

    % of body water measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).

  8. Body composition - fat

    Time frame: in the morning before the test

    % of body fat measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).

  9. Lean body mass

    Time frame: in the morning before the test

    Lean body mass [kg] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).

  10. Height

    Time frame: in the morning before the test

    Prior to the exercise test, we measured the anthropometric parameters, including height (Seca 213 Hamburg, Deutschland) [cm]

  11. Gastrointestinal sypthomps self reported scale

    Time frame: up to 1 hours after the test

    10 point scale were 10 is a outcome of the worst severity

Sponsors and collaborators

Lead sponsor

Poznan University of Physical Education

Other

Registry information

Official study title

Influance of 6000m Ergometer Test on Inflammatory, Permeability Markers and Iron Status.

Acronym: 6000m

Important dates

Study start
2023
Primary completion
2023
Study completion
2023
First posted
Mar 24, 2025
Registry last updated
Mar 27, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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