Implementation of the Methylome in the Characterization of Solid Tumors

A total of 180 cases will be collected: 60 central nervous system tumors, 60 endometrial carcinomas, and 60 endocrine tumors (pituitary tumors, adrenal tumors, thyroid tumors).

By following these practical steps, the project aims to develop and implement a methylation-based tumor classification system that improves clinical decision-making and patient care in the field of solid tumor oncology.

• Study design and sample collection:

• Select a diverse cohort of patients with various types of solid tumors.
• Collect tumor tissue samples for DNA extraction.
• Select the neoplastic area and extract DNA. 2. NGS profiling:
• Analyze molecular alterations using lab-developed NGS panels developed and in use at the IRCCS molecular pathology laboratory (sequencer: S5 Prime - Thermo Fisher Scientific).

• Methylation profile:
• Methylation analysis using ChIP array (Next 550 - Illumina) to generate methylation data.

• Clinical correlation and interpretation of data:
• Analyze the clinical relevance of tumor classification based on methylation profile. Define how these classifications relate to clinical parameters, to treatment outcome and survival 5. Development of diagnostic/therapeutic protocols:
• Development of interdisciplinary protocols to implement methylation profile -based tumor classification in the clinical setting.

Inclusion criteria

• Confirmed diagnosis of one of the following types of cancer: gliomas, malignant thyroid neoplasms, or endometrial carcinomas (according to the WHO Nomenclature)
• Availability of adequate material for analysis at the laboratory, specifically availability of at least 3 sections of 10 µm of FFPE

Exclusion criteria

• no