Impact of Trans Fatty Acids From Natural and Industrial Origin in the Induction of Insulin Resistance Development

Women will be included in the study according to their susceptibility to develop a insulin resistance (evaluated starting from the body index of mass IMC superior to 28 kg.m-², of the waist measurement 88 cm). Once included in the study, the volunteers will receive for 4 weeks butter, dairy products and biscuits enriched in stearic, elaidic or vaccenic acid. The volunteers will undergo a measurement of the body composition by biphotonic absorptiometry as well as a complete metabolic assessment (blood and urinary samples, clamp to insulin and biopsies).

Inclusion criteria

• female
• waist measurement > 88 cm
• body mass index> 28kg/m2
• Affiliated to National Health Insurance
• Subject giving his/her written informed consent
• Subject willing to comply with the study procedures
• Subject considered as normal after clinical examination and medical questionnaire

Exclusion criteria

• Reported food allergies
• Currently participating or having participated in another clinical trial during the last 4 weeks prior to the beginning of this study
• Hepatic or renal impairments
• Positive serologies to HIV or HCV, determined on blood sample
• pregnant or presently attempting to get pregnant or menopause or lactating Blood donation done less than 2 months before the start of the study
• Chronic pathologies: diabetes, hypertriglyceridemia, hypertension, cardiovascular diseases, chronic inflammatory diseases
• Intestine, cardiovascular, kidney and cancer pathologies in the last 5 years
• Previous heavy intestine surgery (except appendicectomy)
• Previous medical and/or surgery judged by the investigator as incompatible with this study
• High variation (> 5%) of body weight during the last 3 months
• Consuming nutritional supplements which could interfered with lipid metabolism (fish oil capsule, vitamins, soja lecithins,…)
• Heavy consumer of alcohol
• Smoker or ex-smoker who stopped smoking less than 1 month before V0 (more than 5 cigarettes/dy).
• Practising intensive physical exercise (> 5 h per week)
• Vegetarian or vegan
• Being under someone's supervision
• Refusal to be registered on the National Volunteers Data file
• Disliking butter, margarine, cheese, biscuits and/or vegetable oil
• Dietary habits unreliable to controlled food intake
• Being in exclusion on the National Volunteers Data file