NC State University
Raleigh, North Carolina, 27606, United States
Completed
NCT Number: NCT07589166
Identifying Terpenoid- and Carotenoid-modifying Enzymes in the Human Gut
In this study, the investigators will collect fecal samples from healthy adults. The investigators will extract DNA from these fecal samples and use microbiological techniques to determine whether this DNA encodes enzymes that can react with certain chemicals called terpenoids and carotenoids. The investigators will additionally collect dietary information via a questionnaire.
Completed
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Conditions
Age range
18 year–65 year
Sex eligibility
All sexes
Study type
Observational
Primary location
Who can participate
Healthy volunteers accepted: Yes
Only the study team can determine whether someone qualifies for participation.
Inclusion criteria
- Body Mass index <25
Exclusion criteria
- Pregnant
- Taken antibiotics or antifungals in the past 3 months
- Taken probiotic supplements in the past 3 months
- Diarrhea in the past month
Treatment and study plan
No Intervention: Observational Cohort
OtherNo intervention
Primary outcomes
-
Number of unique gut metagenomic enzymes with carotenoid-modifying activities for each participant.
Time frame: Day 1
Subjects' fecal metagenomic DNA will be extracted from their fecal sample, and random fragments of this metagenomic DNA will be inserted into an E. coli expression vector. This vector library will be transformed into an E. coli strain that will change color if a carotenoid-modifying gene is present. We expect to obtain about one million E. coli clones per participant. These clones will be spread on agar plates, and the colonies exhibiting different colors will be recovered and sequenced. The sequences of each participant will be collected and annotated to determine putative carotenoid-modifying enzymes, and the number of unique enzymes will be saved.
Sponsors and collaborators
Lead sponsor
North Carolina State University
Other
Collaborators
- National Center for Complementary and Integrative Health (NCCIH)
Registry information
Official study title
Phage-Enabled Mining of Gut Metagenomes for Terpenoid and Carotenoid Metabolism
Acronym: NCState
Important dates
- Study start
- 2025
- Primary completion
- 2026
- Study completion
- 2026
- First posted
- May 15, 2026
- Registry last updated
- May 20, 2026
OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.
View the official ClinicalTrials.gov record (opens in a new tab)This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.