HPV in Blood Samples From Cervical Cancer Patients.

This study hypothesises that in patients with HPV-related cervical cancer, HPV DNA may be released into the bloodstream from tumor cells. These fragments of HPV DNA shed by tumor may therefore be measured in blood samples from the patients. By using the same setting as for real-time PCR with PCR primers and probes for fluorescence detection, the study uses digital droplet PCR (ddPCR), a method based on dilution and partitioning of the blood sample in many reaction chambers or droplets, to measure absolute quantities of HPV DNA fragments in blood samples from women with different stages of cervical cancer. Furthermore, our research group has developed in-house amplicon-based Next Generation Sequencing (NGS) assays for HPV detection and genotyping (the NGS HPV genotyping panel) and HPV integration status and variants (the HPV16 panel), and blood samples are also analysed with the NGS HPV genotyping panel. Cervical tissue samples from primary tumor are collected and analysed with the NGS HPV genotyping panel, and HPV16 positives are further analysed with the NGS HPV16 panel. Patients are recruited at the time of diagnosis, where a baseline blood sample is collected. Follow-up blood samples are collected during treatment and at follow-up visits up to two years after the diagnosis. We expect the HPV DNA load to decrease after treatment, and if an increase in viral load is detected during follow-up, we expect this to be an early sign of a disease recurrence. The method may therefore become an effective monitoring tool in these patients in terms of detecting a ongoing disease recurrence, which gives us the chance to intervene and treat these patients before the disease becomes too disseminated.

For included patients experiencing af recurrence of their disease, tissue samples from recurrent disease are collected and analysed with both the NGS HPV genotyping panel, the NGS HPV16 panel (for HPV16 positives), p16 staining, and a panel detecting relevant somatic mutations in the TP53 and RB1 tumor suppressor proteins.

Inclusion criteria

cases:

• Diagnosed with cervical cancer ≥ stage 1B between june 2018 and june 2020
• > 18 years of age at the time of diagnosis
• There must be available cervical tissue material from the patient to analyse for HPV

Inclusion criteria

healthy controls

• Women > 18 years with no prior history of any cervical dysplasia

Inclusion criteria

CIN3 controls

• Women > 18 years
• Must have a histologically verified severe cervical dysplasia (CIN3)
• Is admitted for cervical conisation

Exclusion criteria

cases:

• < 18 years of age at time of cervical cancer diagnosis
• Cervical cancer < stage 1B

Exclusion criteria

healthy controls:

• < 18 years of age
• Prior cervical dysplasia

Exclusion criteria

CIN3 controls:

• < 18 years of age
• Women with only low grades of cervical dysplasia (CIN1 or CIN2)
• Women with HPV-negative cervical biopsies