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Completed

NCT Number: NCT07598942

Gingival TrxR1 Expression in Periodontal Health and Periodontitis

This study aims to investigate the role of the thioredoxin system in the development and progression of periodontitis. Researchers will examine the levels of specific antioxidant markers, such as thioredoxin reductase 1 (TrxR1), glutathione (GSH), and malondialdehyde (MDA), in gingival tissue samples collected from healthy individuals and patients with different stages of periodontal disease. The goal is to determine if TrxR1 levels can serve as a potential biomarker for diagnosing the severity and activity of periodontitis.

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Key information

Conditions

Periodontitis Mouth Diseases Oxidative Stress Periodontal Diseases Stomatognathic Diseases

Age range

18 year–70 year

Sex eligibility

All sexes

Study type

Observational

Primary location

Atatürk University Faculty of Dentistry

Erzurum, 25240, Turkey (Türkiye)

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Being between the ages of 18 and 70.
  • Having no known systemic disease.
  • Non-smokers.
  • No history of medication or supplement use in the last 3 months.
  • For the Periodontitis groups: Interdental clinical attachment loss (CAL), probing pocket depth (PPD), and radiographic bone loss according to 2017 World Workshop criteria.

Exclusion criteria

  • Smoking.
  • Pregnancy or breastfeeding.
  • Presence of a systemic disease (e.g., diabetes mellitus, hypertension, rheumatoid arthritis, cancer, thyroid disorders, kidney or lung diseases).
  • History of any periodontal surgical procedure within the last 6 months.
  • Use of medication or supplements within the last 3 months.

Treatment and study plan

Gingival Tissue Analysis

Other

Gingival tissue samples are collected from all participants. These samples are analyzed for Glutathione (GSH) and Malondialdehyde (MDA) levels using spectroscopic methods. Additionally, the expression of thioredoxin reductase 1 (TrxR1), thioredoxin (TXN), and thioredoxin-interacting protein (TXNIP) is determined through quantitative real-time PCR, western blot, and enzyme activity assays.

Primary outcomes

  1. Thioredoxin Reductase 1 (TrxR1) mRNA Expression Levels

    Time frame: Baseline (at the time of tissue collection)

    The expression levels of TrxR1 in gingival tissue are determined by quantitative real-time PCR (qPCR). This measure evaluates the potential of TrxR1 as a biomarker for periodontitis severity.

Secondary outcomes

  1. Thioredoxin (TXN) mRNA Expression Levels

    Time frame: Baseline (at the time of tissue collection)

    Evaluation of TXN gene expression levels in gingival tissue samples using quantitative real-time PCR (qPCR) normalized to β-actin.

  2. Thioredoxin-Interacting Protein (TXNIP) mRNA Expression Levels

    Time frame: Baseline (at the time of tissue collection)

    Evaluation of TXNIP gene expression levels in gingival tissue samples using quantitative real-time PCR (qPCR) normalized to β-actin.

    Time Frame: Baseline (at the time of tissue collection)

  3. Malondialdehyde (MDA) Levels.

    Time frame: Baseline (at the time of tissue collection).

    MDA levels in gingival tissue homogenates measured using the thiobarbituric acid reactive substances (TBARS) assay to evaluate lipid peroxidation.

  4. Glutathione (GSH) Levels.

    Time frame: Baseline (at the time of tissue collection).

    GSH levels in gingival tissue homogenates measured using spectroscopic methods to evaluate antioxidant status.

Sponsors and collaborators

Lead sponsor

Ataturk University

Other

Registry information

Official study title

Thioredoxin Reductase 1 Levels in Gingival Tissue as an Oxidative Stress Biomarker in Periodontitis

Important dates

Study start
2023
Primary completion
2025
Study completion
2025
First posted
May 20, 2026
Registry last updated
May 20, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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