Gingival Crevicular Fluid Levels of Protein Carbonyl Following the Use of Lycopene in Chronic Periodontitis

The study was designed as a randomized controlled clinical trial. Total subjects selected were allocated into three study groups, Group I (Test group), Group II (Positive control group) and Group III (Negative control group). Group I: Included 8 patients with severe chronic periodontitis managed by Scaling and root planing and local delivery of lycopene loaded in solid lipid microparticles. Group II: Included 8 patients with severe chronic periodontitis managed by Scaling and root planing only. Group III: Included 8 patients with clinically healthy periodontium.

Protein carbonyl biomarker level and clinical parameters were assisted to evaluate the effect of lycopene as an adjunct to scaling and root planing.

Inclusion criteria

• All subjects were free from any systemic diseases.
• Subjects for group I and II were suffering from severe chronic periodontitis (Armitage et al., 1999).
• Group III included subjects with clinically healthy periodontium.
• All subjects were between 35-60 years old.
• Both genders were included.
• Subjects were able to return for follow up visits.
• Subjects agreed to sign a written consent after the nature of the study was explained.

Exclusion criteria

• History of scaling and root planing or periodontal surgery < 6 months prior to initiation of the study.
• History of administration of antimicrobial drugs or over the counter antioxidants like Vit C, Vit B, β-carotene < 6 months prior to the baseline examination.
• Subjects who reported any side effects or drug allergies from antioxidants.
• Subjects with mobile teeth, abscesses and defected restorations related to the affected sites.
• Subjects with para-functional habits.
• Pregnant and lactating females.
• Smokers, alcoholic and drug abusers.
• Prisoner.
• Mentally retarded patients.