Genotype and Subtype Mapping of the Hepatitis C Virus

The study will consist of the following:

• Inclusion of patients who meet the selection criteria (hospitalized patients and outpatients from tertiary centers in mainland France) ;
• Collecting all associated data, including demographic, clinical, and therapeutic information. A Clinical research technician may be assigned to centers lacking sufficient human resources;
• Collect leftover patient specimens for viral genotyping (phylogenetic analysis of a portion of the NS5B gene, the reference method) or complete genome sequencing of unusual subtypes;
• Perform molecular biology genotyping techniques (RNA extraction, PCR, and Sanger sequencing of a portion of the NS5B gene);
• Carry out next-generation sequencing (Seq2000, Illumina) of the full genome of "unusual" subtype strains, or DAAs resistant patients
• Analyze genotype and subtype information, as well as its correlation with demographic, clinical, and therapeutic data.

Inclusion criteria

• Patient aged 18 years old or more
• Newly diagnosed patient positive for HCV (presence of HCV antibodies)
• Patient with active infection (HCV RNA > 0)

Exclusion criteria

• Patients who decline to participate in the study and oppose the use of their personal data
• Patients under legal protection (guardianship or curatorship)