Karolinska Sjukhuset Huddinge
Huddinge, Stockholm County, Sweden
Completed
NCT Number: NCT06484101
Functional Characterization of CD8+ T Cells After Supramaximal Exercise
Healthy human subjects perform supramaximal exercise with venous blood samples obtained before and up to 60 minutes after the exercise bout.
Completed
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Age range
20 year–36 year
Sex eligibility
All sexes
Study type
Interventional
Phase
Not applicable
Primary location
About this study
Healthy, human subjects perform 3 x 30 seconds of supramaximal exercise. Venous blood samples were obtained before and up to 60 minutes after the exercise bout. The blood was used for absolute count of lymphocytes and monocytes per volume blood, and for isolation of CD8+ T cells. It was also used for blood gas analysis. The CD8+ T cells were characterized using flow cytometry, and then cultured for 7 days with CD8+ activation beads. The cells were counted and analyzed for viability at day4 and day8. RNAseq was performed on CD8+ cells isolated before and directly after exercise. CD8+ T cells from Day 1 pre and post exercise and then after 7 days of culture, were analyzed for IFNgamma release using Elispot, and for cytotoxicity against HEK cancer cells in vitro. Flow cytometry of CD8+ T cells was performed on fresh CD8 T cells pre and post exercise, and then on day4 and day8 after culture. SeaHorse metabolic assay was done on Day1 CD8 T cells obtained pre and post exercise.
Who can participate
Healthy volunteers accepted: Yes
Only the study team can determine whether someone qualifies for participation.
Inclusion criteria
- Be used to physical exercise and be able to perform strenous exercise Be able to attend the experimental procedure for the 90 minute duration of the study
Exclusion criteria
- Smoking Pregnant Have any medical condition requiring medical treatment
Treatment and study plan
Exercise
Procedure3 x 30 seconds of supramaximal exercise
Primary outcomes
-
CD8 T cell absolute nr after exercise
Time frame: Before, directly after, 60 minutes after exercise
CD8 nr
-
CD8 T cell cytotoxicity against cancer cells
Time frame: Cryopreserved CD8 cells from Day 1 and Day 8 of the experiment, obtained before and after exercise
CD8 T cell lysis of cancer cells
-
CD8 T cell pheontype after exercise
Time frame: Before, directly after, 30 minutes and 60 minutes after exercise
Flow cytometry analysis of CD8 T cells
Sponsors and collaborators
Lead sponsor
Karolinska Institutet
Other
Registry information
Official study title
Effekten av högintensiv träning på Vita Blodkroppar, Med Speciellt Fokus på Funktion Kopplad Till Att bekämpa Cancerceller
Important dates
- Study start
- 2023
- Primary completion
- 2023
- Study completion
- 2024
- First posted
- Jul 3, 2024
- Registry last updated
- Jul 3, 2024
OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.
View the official ClinicalTrials.gov record (opens in a new tab)This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.