NCT Number: NCT05670301
A multi-centre, prospective study to study cytokine profiles and other potential disease-specific biomarkers in patients with presumed or confirmed diseases of systemic inflammation
The goal of this prospective, observational study is to describe the longitudinal evolution of blood cytokine profiles in patients with presumed or confirmed diseases of systemic inflammation
The main questions it aims to answer are:
* What are the differences and similarities in blood cytokines between different patients and groups presenting symptoms of systemic inflammatory conditions? * How is the cytokine profile of individual patients evolving over time and what is the effect of different therapeutics? * Is cytokine profiling a valuable tool to diagnose and follow-up on patients with systemic inflammatory conditions?
Participants will be asked to give an additional blood volume for research purposes when blood sampling is performed for routine clinical purposes. A subset of patients (those initiated on biologicals) will also be asked to complete questionnaires.
Researchers will compare the blood cytokines profiles between the different groups of systemic inflammatory conditions and with healthy individuals.
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Interventional
Not applicable
UZ Antwerpen, Antwerp, Belgium
FEBRIS is a prospective, observational and multi-centric study involving 5 hospitals in Flanders, Belgium.
In this study, the researchers will us ehe FEBRIS Cytokine Profile Assay, which is a multiplex assay measuring pro- and anti-inflammatory cytokines in the blood of subjects. It has been developed in a retrospective cohort of human patients presenting systemic inflammatory disorders.
With this study, the researchers aim to prospectively validate the use of this assay in a cohort of patients that present signs reminiscent of systemic inflammation, including patients with antoinflammatory conditions, autoimmune disorders, hyperinflammatory syndromes (e.g. hemophagocytic lymphohistiocytosis and macrophage activation syndrome) and systemic infection. In parallel, serum amyloid A will be quantified in all patients. Lipidomics will be performed on patients with sufficient leftover sample. The researchers will integrate the data from these assays with clinical parameters at baseline and during follow-up to correlate markers with the clinical or genetic diagnosis, prognosis and effect of different therapeutics.
Patients fulfilling the inclusion criteria are eligible for longitudinal blood sampling (every 3-6 months) during the study period (maximum of 4 years). Additional clotted blood will only be collected when a blood sample for routine clinical purposes is performed.
In addition, patients initiated on biological therapeutics will be asked to complete questionnaires regarding health-related quality of life to document outcome and improve rational and cost-efficient use of additional investigations and treatment.
Healthy volunteers accepted: Yes
Only the study team can determine whether someone qualifies for participation.
Inclusion criteria
An individual who meets any of the following criteria can be eligible for participation in this study:
Exclusion criteria
An individual who meets any of the following criteria will be excluded from participation in this study:
Besides the above mentioned patients, volunteers that meet the requirements as a healthy individual are eligible for inclusion as controls.
Time frame: full study period (up to 48 months)
On the sera of patients, the FEBRIS Cytokine Profile Assay will be performed.
The FEBRIS Cytokine Profile Assay is a multiplex panel of inflammatory cytokines (interleukin (IL) 1 beta, IL-1RA, IL-6, IL-18, tumor necrosis factor (TNF) alpha, CXCL9 and CXCL10) that will be assessed by Meso Scale Discovery (MSD) technology. The concentration of cytokines will be quantified in pg/ml.
Sera will be analyzed upon entry in the study and during follow-up (minimum interval of 3 months between samples).
Data analyses (multiple logistic regression) will be performed on the cytokine fingerprints of different patient groups taking clinical control of patients into account (sampling during a flare of inflammation versus sampling during disease remission).
Time frame: full study period (up to 48 months)
In patients included in the study that are initiated on biopharmaceuticals, additional blood sampling will be carried out before start of treatment and after 3 months of therapy. On this blood the FEBRIS Cytokine Profile Assay (measurement of IL-1 beta, IL-1RA, IL-6, IL-18, TNF alpha, CXCL9 and CXCL10) will be performed. The concentration of cytokines will be quantified in pg/ml.
This data will be used in retrospective analyses with the aim to identify biomarkers or cytokine profiles that separate responders from non-responders.
Time frame: full study period (up to 48 months)
At each study visit, the patient's age will be registered through a structured case report form.
Age will be registered in years or in months if the child is younger than 2 years.
Time frame: full study period (up to 48 months)
At each study visit, the patient's biological sex will be registered through a structured case report form.
Sex will be registered as female, male or intersex
Time frame: full study period (up to 48 months)
At each study visit, the patient's body weight will be registered in kg.
Time frame: full study period (up to 48 months)
At each study visit, the patient's length will be registered in cm.
Time frame: full study period (up to 48 months)
At each study visit, the patient's known (clinically or genetically confirmed) or tentative inflammatory disease will be registered. This will be registered through a multiple choice list including autoinflammatory disorders (familial Mediterranean fever, tumor necrosis factor receptor associated periodic syndrome, cryopyrin-associated autoinflammatory syndromes, mevalonate kinase deficiency, systemic onset juvenile idiopathic arthritis, periodic fever adenitis pharyngitis and aphthosis, Aicardi-Gutieres syndrome), autoimmune disorders (systemic lupus erythematosus, dermatomyositis, rheumatoid arthritis, ankylosing spondylitis, psoriatic arthritis, oligoarthritic juvenile idiopathic arthritis) or hyperacute inflammatory disease (hemophagocytic lymphohistiocytosis, macrophage activation syndrome, infectious disease related cytokine storm) or other and unspecified inflammatory syndromes.
Time frame: full study period (up to 48 months)
At each study visit, the patient's clinical status will be registered through a multiple-choice list to register the presence or absence of symptoms currently or in the 48 hours before blood sampling. Symptoms include fever, hepatomegaly, splenomegaly, myalgia, arthralgia, exanthema, pharyngitis, oral aphthosis, lymphadenopathy, abdominal pain, thoracic pain, enthesitis.
Time frame: full study period (up to 48 months)
At each study visit, the patient's current medication will be registered through a multiple-choice list to register the use of therapeutics that have an effect on inflammation. These include systemic non-steroidal anti-inflammatory drugs, systemic corticosteroids, colchicine, anakinra, canakinumab, tocilizumab, TNF inhibitors, hydroxychloroquine, mycophenolate, methotrexate, janus kinase (JAK) inhibitors, abatacept.
Time frame: full study periode (up to 48 months)
In the subgroup of patients initiated on biopharmaceuticals, health-related costs will be documented.
To document costs, a questionnaire will be used that was drafted specifically for this project, incorporating registration of the number of visits with caregivers, the type and use of medication, the number and duration of hospitalizations, and questions relating to absence from work.
A health economic specialist will aggregate the data from this questionnaire to calculate an annual cost in euros per patient.
This questionnaire will be given to the patients at initiation of biopharmaceutical treatments and every 6 months throughout the study period.
Time frame: full study periode (up to 48 months)
In the subgroup of patients initiated on biopharmaceuticals, health-related quality of life (HRQoL) scores will be documented.
To document HRQoL, the translated version of the PEDsQL and short form 36 (SF-36) will be used for children and adults, respectively.
The raw scores of PEDsQL are converted into three scale scores: a total score, a psychosocial health score and a physical health score.
For the SF-36, the item scores are summed into scale scores (weighted sums) and transformed to a 0-100 scale (each question carries equal weight).
This questionnaire will be given to the patients at initiation of biopharmaceutical treatments and every 6 months throughout the study period.
Contact information is provided by the study sponsor or research team.
University Hospital, Ghent
Other
Acronym: FEBRIS
OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.
View the official ClinicalTrials.gov record (opens in a new tab)This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.
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