School of Kinesiology and Health Studies
Kingston, Ontario, K7L3N6, Canada
Completed
NCT Number: NCT03811717
Fasting and Exercise-induced Changes in Human Skeletal Muscle
This study examines the impact of increasing energetic stress during an 8-hour fast on metabolic pathways in human skeletal muscle. Specifically, participants will undergo two 8-hour fasting sessions in a randomized crossover fashion, with the addition of low-intensity arm exercise in one of the sessions. Changes in metabolic pathways will be examined in resting (leg) muscle before, during, and after the fast.
Completed
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Notify MeKey information
Conditions
Age range
18 year–30 year
Sex eligibility
Male
Study type
Interventional
Phase
Not applicable
Primary location
Who can participate
Healthy volunteers accepted: Yes
Only the study team can determine whether someone qualifies for participation.
Inclusion criteria
- recreationally active (no more than 3 hours of weekly physical activity)
- not currently involved in a systematic training program
- body mass index < 30 kg/m2
Exclusion criteria
- presence of cardiometabolic disease
- taking regular oral medication
- current smoker
Treatment and study plan
FAST+EX
OtherParticipants will perform 2-hours of low-intensity arm exercise (8 x 15 min bouts of arm ergometer exercise separated by 10 min of rest) during an 8-hour fast.
FAST
OtherParticipants will complete an 8-hour fast.
Primary outcomes
-
Peroxisome proliferator activated receptor gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression
Time frame: 15 min before fast; after 4 and 8 hours of fasting
PGC-1α gene expression (indirect indicator of PGC-1α activity)
Secondary outcomes
-
Catecholamine concentrations
Time frame: 15 min before fast; after exercise; after 4 and 8 hours of fasting
Epinephrine and norepinephrine concentrations in plasma samples
-
Glucose and insulin concentrations
Time frame: 15 min before fast; after 4 and 8 hours of fasting
Glucose and insulin concentrations in plasma samples
-
total and phosphorylated AMP activated protein kinase (AMPK)
Time frame: 15 min before fast; after 4 and 8 hours of fasting
active AMPK relative to total AMPK protein content
-
total and phosphorylated SIRT-1
Time frame: 15 min before fast; after 4 and 8 hours of fasting
active SIRT-1 relative to total SIRT1 protein content
-
total and phosphorylated mammalian target of rapamycin (mTOR)
Time frame: 15 min before fast; after 4 and 8 hours of fasting
active mTOR relative to total mTOR protein content
-
total and phosphorylated Akt/Protein Kinase B
Time frame: 15 min before fast; after 4 and 8 hours of fasting
active Akt relative to total Akt protein content
-
total and phosphorylated acetyl coenzyme A carboxylase (ACC)
Time frame: 15 min before fast; after 4 and 8 hours of fasting
active ACC relative to total ACC protein
-
total and phosphorylated p38 mitogen activated protein kinase (MAPK)
Time frame: 15 min before fast; after 4 and 8 hours of fasting
active p38 MAPK relative to total p38 MAPK protein
-
phosphorylated protein kinase A (PKA) substrates
Time frame: 15 min before fast; after 4 and 8 hours of fasting
phosphorylated PKA substrates (indirect measure of PKA activity)
-
acetylated p53
Time frame: 15 min before fast; after 4 and 8 hours of fasting
p53 acetylation status (indirect measure of SIRT-1 deacetylase activity)
-
Sirtuin 1 (SIRT1) mRNA expression
Time frame: 15 min before fast; after 4 and 8 hours of fasting
gene expression of the deacetylase enzyme SIRT1
-
Sirtuin 3 (SIRT3) mRNA expression
Time frame: 15 min before fast; after 4 and 8 hours of fasting
gene expression of the deacetylase enzyme SIRT3
-
Pyruvate dehydrogenase kinase isoenzyme 4 (PDK4) mRNA expression
Time frame: 15 min before fast; after 4 and 8 hours of fasting
gene expression of the PGC-1α target PDK4
-
p53 mRNA expression
Time frame: 15 min before fast; after 4 and 8 hours of fasting
gene expression of the tumour suppressor protein p53
-
Leucine rich pentatricopeptide repeat containing protein (LRP130) mRNA expression
Time frame: 15 min before fast; after 4 and 8 hours of fasting
gene expression of RNA-binding protein LRP130
-
General control non-repressible 5 (GCN5) mRNA expression
Time frame: 15 min before fast; after 4 and 8 hours of fasting
gene expression of the acetyltransferase GCN5
-
PGC-1α protein content
Time frame: 15 min before fast; after 4 and 8 hours of fasting
Protein content of the transcriptional co-activator PGC-1α
-
Cytochrome C oxidase subunit 4 (COX-IV) protein content
Time frame: 15 min before fast; after 4 and 8 hours of fasting
Protein content of the mitochondrial enzyme COX subunit 4
-
Intramuscular glycogen content
Time frame: 15 min before fast; after 4 and 8 hours of fasting
Muscle glycogen content
-
Intramuscular triglyceride (IMTG) content
Time frame: 15 min before fast; after 4 and 8 hours of fasting
Muscle IMTG content
Sponsors and collaborators
Lead sponsor
Queen's University
Other
Collaborators
- University of Waterloo
Registry information
Official study title
Impact of Exercise-induced Energetic Stress During an 8-hour Fast on Metabolic Regulation in Resting Human Skeletal Muscle
Important dates
- Study start
- 2019
- Primary completion
- 2019
- Study completion
- 2019
- First posted
- Jan 22, 2019
- Registry last updated
- May 30, 2019
OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.
View the official ClinicalTrials.gov record (opens in a new tab)This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.