Exploratory Study of Intrapulmonary Microdosing of Gram-negative Optical Imaging Detection Probe

The primary objective of this study is to deliver a BAC TWO microdose to 3 ventilated controls and 9 patients to assess the imaging parameters of BAC TWO over human autofluorescence and to assess if gram-negative bacteria can be detected in vivo in situ within the distal lung. The primary endpoint is to visualise the delivery of a microdose of BAC TWO and assess imaging parameters in;

• 3 mechanically ventilated patients to provide a control population (cohort 1)
• 6 bronchiectasis patients with predominant colonisation with gram-negative bacteria (cohort 2)
• 6 bronchiectasis patients with predominant colonisation with gram-positive bacteria (cohort 3)
• 3 patients with suspected pneumonia and pulmonary infiltrates in ICU (cohort 4)

For all cohorts, eligibility will be verified by a clinical trial physician after written informed consent has been obtained. For all cohorts, a bronchoscopy with lavage will be performed to harvest broncho-alveolar lavage fluid (BALF). Fibre-based endomicroscopy (FE) will be performed on up to three areas and up to 80μg (± 25%) in total of BAC TWO will be instilled in up to 3 sites.

Participants will be asked to provide additional blood and urine samples with the intention of examining for systemic uptake of the BAC TWO probe. Routine blood investigations will be performed 4-6 hours following the administration of BAC TWO. The completion of all assessments at 4-6 hours post dose marks the end of the participant's participation in this study unless there are ongoing adverse events requiring resolution. The primary aim will be measured during bronchoscopy and all routine investigations will have been completed 6 hours post dose.

Inclusion criteria

All cohorts

• ≥ 16 years
• Attending consultant permission for bronchoscopy

Cohort 1

• Patients scheduled to undergo surgery under general anaesthesia
• Absence of acute or significant chronic lung disease as determined by the clinical suspicion of the attending medical team or of a medically qualified member of the study investigation team.
• Presence or scheduled presence of endo-tracheal tube.
• Capacity to provide informed consent

Cohort 2 and 3

• Patients with bronchiectasis with known microbiological predominance of gram-negative or gram-positive bacteria.
• Capacity to provide informed consent

Cohort 4

• Patients in the ICU with pulmonary infiltrates on radiological assessment
• Presence of invasive tracheal ventilation tube
• Provision of informed consent from the patient or their personal legal representative prior to any study related procedures.

Exclusion criteria

All cohorts

• Refusal for participation by attending consultant
• Any history of anaphylaxis or allergy to polymyxin-based antibiotics e.g. colomycin
• Significant coagulopathy, which causes bronchoscopy to be unsuitable, as determined by clinical co-investigator or the participant's attending consultant, using information which is routinely available
• Myocardial infarction in the preceding four weeks
• Women who are pregnant or are breastfeeding
• Receiving drugs that cause increased autofluorescence in the lung, specifically amiodorane and methotrexate

Cohort 4 only

• Inspired Oxygen Concentration (FiO2) >70%
• Positive End Expiratory Pressure (PEEP) >10cm
• Endotracheal tube (ETT) or tracheostomy internal diameter < 7mm
• Presence of pneumothorax
• Active bronchospasm
• Mean arterial pressure <65mmHg (millimeter of mercury) AND on vasopressor
• Platelet count < 50 x 109/L