Skip to main content
OpenTrials
Completed

NCT Number: NCT02456870

Expandability of Aging Adipose Tissue

The purpose of this pilot study is to determine whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. The investigators hypothesize that the adipose tissue response to overfeeding may be weakened in older when compared to younger men, thereby leading to worsened ectopic storage depots for older men.

Completed

Looking for future studies?

Notify Me

Key information

Conditions

Aging Body Weight Nutrition Disorders Nutritional and Metabolic Diseases Obesity Overnutrition Overweight Pathological Conditions, Signs and Symptoms Signs and Symptoms

Age range

25 year–75 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Univeristy of Colorado, Anschutz Medical Campus

Aurora, Colorado, 80045, United States

About this study

This pilot study will begin to address whether adipose tissue biology changes with advancing age. Specifically, we will test whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. To test this possibility the Investigators will recruit overweight and mildly obese older (age 55-75y) and younger (age 25-40y) men and collect adipose tissue biopsies before and after 7 days of overfeeding. The investigators will test how well the cells in adipose tissue expand in response to overfeeding and whether this differs between younger and older men.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Men
  • Aged 25-40y OR 55-75y
  • BMI 28-35 kg/m2
  • Weight stable (±2kg over past 3 mo)
  • Sedentary to moderately active (<3 d/wk of exercise lasting >20min)
  • Non-smokers (never used or quit >7yrs prior)

Exclusion criteria

  • Women
  • Currently using hormones, glucose or lipid lowering medication
  • Engaged in exercise or diet program
  • Currently gaining or losing weight or using weight loss drugs
  • Diabetes mellitus (diagnosis, treatment, FBG ≥126 mg/dL)
  • Uncontrolled metabolic disorders (e.g., thyroid)

Treatment and study plan

Overfeeding

Other

Consumption of 40% excess calories for 7 consecutive days

Primary outcomes

  1. Change in adipose-derived stem cell (ASC) differentiation (Delta%oil red O+)

    Time frame: 1 wk of overfeeding

    age-related difference in the ASC differentiation response to overfeeding

Secondary outcomes

  1. Change in ASC proliferation (Delta rate of cell growth)

    Time frame: 1 wk of overfeeding

    age-related difference in the ASC proliferative response to overfeeding

  2. Change in expression of C/EBPalpha, C/EBPbeta, PPARgamma2, SREBP1c, SREBf1a, and Wnt10b (fold-changes in mRNA expression)

    Time frame: 1 wk overfeeding

    age-related difference in the pro-adipogenic response to overfeeding

  3. Change in intramyocellular (IMCL) di-saturated diacylglycerol (DAG) localization (Delta %membrane:cytosol)

    Time frame: 1 week overfeeding

    age-related difference in the IMCL DAG response to overfeeding

Sponsors and collaborators

Lead sponsor

University of Colorado, Denver

Other

Collaborators

  • Colorado State University

Registry information

Official study title

Aging Subcutaneous Adipose Tissue Response to Overfeeding

Acronym: EAT

Important dates

Study start
2015
Primary completion
2016
Study completion
2018
First posted
May 29, 2015
Registry last updated
Jun 13, 2019

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Related clinical trials

Published trials that share one or more normalized conditions with this study.