Skip to main content
OpenTrials
Completed

NCT Number: NCT05616234

Exercise-induced Changes in Exosomes

The proposed study aims to shed light on the function/importance/relevance of exercise-induced changes in exosomes to connective tissues. Exosomes are known to increase robustly in response to exercise. We have previously shown that serum isolated from subjects after they lift heavy weights increases human engineered ligament collagen content and mechanics more than serum from before they lift weight. Further, we showed that exercise-induced changes in hormones could not explain the change in ligament structure or function. These data indicate that there is a significant gap in our understanding of muscle-connective tissue crosstalk. To address this gap, the current proposal seeks to: i) isolate and sequence exosomal RNA (long non-coding, miR, and mRNA) and ii) determine whether exosomes isolated from serum after exercise increase engineered ligament mechanics and collagen content.

Completed

Looking for future studies?

Notify Me

Key information

Conditions

Exosomes Behavior Connective Tissue Exercise Motor Activity

Age range

18 year–35 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Hickey Laboratory

Davis, California, 95616, United States

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Young healthy adults (18-35 y)
  • Experience with exercise (>1y)

Exclusion criteria

  • Pregnancy
  • Smoking
  • Receiving any medication that may interfere with the study outcomes

Treatment and study plan

Resistance Exercise

Procedure

Participants will perform a bout of lower body resistance exercise consisting of leg press, leg extension and leg curls.

Primary outcomes

  1. Collagen content of ligament constructs

    Time frame: 15 min post-exercise

    Ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of collagen content using a hydroxyproline assay.

Secondary outcomes

  1. Ultimate tensile stress and modulus of ligament constructs

    Time frame: 15 min post-exercise

    ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of ultimate tensile stress and modulus.

  2. Exosomal RNA

    Time frame: 15 min post-exercise

    Isolation and sequencing of exosomal RNA from serum obtained at baseline or post-exercise.

Sponsors and collaborators

Lead sponsor

University of California, Davis

Other

Registry information

Important dates

Study start
2022
Primary completion
2023
Study completion
2023
First posted
Nov 15, 2022
Registry last updated
Jun 15, 2023

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Related clinical trials

Published trials that share one or more normalized conditions with this study.