Evaluation of Interest Chemo-oncogramme in Patients With Colonic Adenocarcinoma Stage 4

A fragment of colon tumors will be taken from a specimen or a cold biopsy, tissue from patients diagnosed with colon carcinoma metastatic (stage 4). In parallel and systematically: analysis by the pathologist of a histological section.

• Culturing the cells obtained from the tumor by Oncomedics fragment in a defined medium and provided for enriching tumor cells compared to stromal cells.
• Maintaining the cells in culture for 7 days.
• Effects of conventional chemotherapy (corresponding to protocols) on these cells for 72 hours (growth chambers) by Oncomedics (chimio-oncogramme).
• For each chemotherapy tested, measuring the proportion of dead cells / total cells.
• Retained by Oncomedics results, not given to the nursing team. The patient will be treated and followed up in the usual way.
• At the end of the study, comparing the in vitro results (chimio-oncogramme) to the patient's response to (x) the same (s) chemotherapy (s).

Inclusion criteria

• patient with adenocarcinoma of the colon diagnosed at stage 4 receiving standard therapy
• targets must be measurable tumor lesions (RECIST)
• patient has not expressed opposition to the use of their residual tumor

Exclusion criteria

• Against-formal indication to an indispensable para-clinical examination of the patient monitoring
• Use of chemotherapy, hormone therapy or concomitant radiotherapy
• Cons-indication for chemotherapy
• Are pregnant, nursing or lack of contraception for women of childbearing age.