Estimation of Level of ERAP1 Expression in Lesional and Non Lesional Skin of Psoraitic Patients and Healthy Controls.

A 4mm Punch biopsy is obtained from lesional and non lesional skin of 25 psoraitic patients as well as of 30 controls.

Skin biopsy from patients (psoriasis lesional and non lesional) & control will be kept in lysis solution (which contains RNAase inhibitor) for the stability of the parameter to be assessed. ERAP-1 gene expression will Research Template 5 Final Version: 8/3/2 016 be detected by quantitative real time -PCR, which include RNA extraction, cDNA synthesis & PCR.

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Key information

Conditions

Psoriasis Skin Diseases Skin Diseases, Papulosquamous Skin and Connective Tissue Diseases

Age range

18 year–75 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

patients with psoriasis vulgaris
patients over 18 years of age
both sexes

Exclusion criteria

patients on treatment for psoriasis within the last two months
assoiciated autoimmune diseases eg SLE
pregnancy

Treatment and study plan

RT-PCR for ERAP1 detection

Diagnostic Test

Reverse transcriptase -Polymerase Chain Reaction for ERAP1 detection

Primary outcomes

Estimation of lesional ERAP1 in psoriatic skin

Time frame: Maximum 2 years

DPCRetection of level of expression of ERAP 1 in lesional skin of psoriasis patients by RT-

Secondary outcomes

Estimation of non lesional ERAP1 in psoriatic skin

Time frame: Maximum 2 years

Detection of level of expression of ERAP 1 in non lesional skin of psoriasis patients by RT-PCR.

Sponsors and collaborators

Lead sponsor

Kasr El Aini Hospital

Other

Registry information

Important dates

Study start: 2017
Primary completion: 2017
Study completion: 2018
First posted: Dec 6, 2018
Registry last updated: Dec 6, 2018

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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Completed