Medical University of Sofia, Faculty of Dental Medicine
Sofia, 1431, Bulgaria
NCT Number: NCT07493278
This study compared two standard methods for minimally invasive caries removal in children: conventional mechanical excavation and Er,Cr:YSGG laser preparation. The study aimed to evaluate the reduction of Streptococcus mutans and Lactobacillus spp. in dentin using PCR analysis. All procedures were performed as part of routine dental care, and written informed consent was obtained from parents or legal guardians.
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Notify Me8 year–15 year
All sexes
Interventional
Not applicable
Sofia, 1431, Bulgaria
This comparative clinical study included 32 carious lesions in permanent molars and premolars from children aged 8-15 years. Lesions were randomly assigned to either conventional mechanical preparation (n=17) or Er,Cr:YSGG laser preparation (n=15). Samples of carious and affected dentin were collected before and after preparation, and bacterial DNA was analyzed using PCR for S. mutans and Lactobacillus spp. The study followed ethical approval from the Ethics Committee of the Medical University of Sofia (protocol № 14/28.09.2023), and written informed consent was obtained from all parents/guardians. The study was conducted entirely within the framework of routine dental treatment, with standard, clinically approved methods applied for all participants. Statistical analysis included descriptive statistics, Wilcoxon signed-rank test for within-group comparisons, and Mann-Whitney U test for between-group comparisons.
Healthy volunteers accepted: No
Only the study team can determine whether someone qualifies for participation.
Inclusion criteria
Exclusion criteria
Participants received conventional mechanical caries removal according to standard pediatric dentistry protocols. Laboratory personnel analyzing PCR results were blinded to group assignments.
Participants received caries removal using the Er,Cr:YSGG laser (2780 nm) according to manufacturer-recommended parameters for minimally invasive dentin excavation. Laboratory personnel analyzing PCR results were blinded to group assignments
Time frame: Before and immediately after caries removal
The presence of S. mutans and Lactobacillus spp. in dentin samples will be assessed using PCR. Samples are collected before and after mechanical or Er,Cr:YSGG laser caries removal to determine bacterial reduction for both microorganisms.
Time frame: Before and immediately after caries removal
The presence of S. mutans and Lactobacillus spp. in dentin samples will be assessed using PCR. Samples are collected before and after mechanical or Er,Cr:YSGG laser caries removal to determine bacterial reduction for both microorganisms.
Medical University of Sofia
Other
Er,Cr:YSGG Laser Versus Conventional Preparation for Minimally Invasive Caries Removal: Clinical PCR Study of Streptococcus Mutans and Lactobacillus Spp. in Dentin Reference to the Main Project (Sub-study of Strategic Research and Innovation Program for Development of Medical University - Sofia, BG-RRP-2.004-0004-C01)
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