Epigenetic Reprogramming of Monocytes in Patients With Coronary Atherosclerosis

Rationale: The innate immune system plays a pivotal role in the development and progression of atherosclerosis. Recently, it was reported that monocytes can develop a long-lasting immunological memory after stimulation with various microorganisms, which has been termed 'trained innate immunity'. This memory is induced by epigenetic reprogramming. We hypothesize that trained monocytes augment atherogenesis.

Objective: The main objective is to study whether patients with coronary atherosclerosis show specific epigenetic changes in the promoter regions of pro-inflammatory cytokines and chemokines and whether this correlates with the inflammatory phenotype of these cells.

Study design: Observational study

Study population: Adult patients who have had computed tomography coronary angiography because of chest pain: 20 patients without atherosclerosis and 20 patients with severe atherosclerosis will be included.

Inclusion criteria

• Patients (male/female),
• aged >18 years,
• admitted to the acute coronary unit of the CWZ hospital for evaluation of chest pain.
• No coronary atherosclerosis
• Or Severe atherosclerosis

Exclusion criteria

• Signs or symptoms of a current infection (fever, chills)
• Auto-immune diseases (such as rheumatoid arthritis)