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NCT Number: NCT07540624

Efficacy of Chlorhexidine and Morus Nigra Mouthwashes After Periodontal Treatment

This study aims to evaluate and compare the clinical, biochemical, and microbiological effects of chlorhexidine and Morus nigra (blackberry) mouthwashes used as an adjunct to non-surgical periodontal treatment (scaling and root planing - SRP) in patients with periodontal diseases. The participants will be divided into three groups: a control group receiving only SRP, a group receiving SRP combined with chlorhexidine mouthwash, and a group receiving SRP combined with Morus nigra mouthwash. Clinical periodontal parameters, gingival crevicular fluid levels of inflammatory markers (including TNF-a, IL-1b, and IL-10), and subgingival plaque microbiological profiles (such as red complex bacteria and Fusobacterium) will be evaluated at baseline, 1 month, and 3 months after the treatment.

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This study is active but is not currently recruiting participants.

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Key information

Conditions

Periodontitis Mouth Diseases Periodontal Disease Periodontal Diseases Stomatognathic Diseases

Age range

18 year–65 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Inonu University Faculty of Dentistry

Battalgazi, Malatya, 44300, Turkey (Türkiye)

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Patients diagnosed with periodontitis.
  • Systemically healthy individuals.
  • Patients aged between 18 and 65 years.
  • Presence of at least 20 natural teeth.
  • Volunteering to participate in the study and signing the informed consent form.

Exclusion criteria

  • Presence of any systemic disease that may affect periodontal health or systemic inflammatory response (e.g., Diabetes Mellitus, immunological disorders).
  • Current smokers or use of any tobacco products.
  • Pregnancy or lactation.
  • Use of systemic antibiotics, anti-inflammatory drugs, or immunosuppressive medications within the last 6 months.
  • Having received any non-surgical or surgical periodontal treatment within the last 6 months.
  • Known allergy or sensitivity to chlorhexidine or Morus nigra (blackberry) extracts.
  • Patients using orthodontic appliances.

Treatment and study plan

0.12% Chlorhexidine Gluconate Mouthwash

Drug

As an adjunct to SRP, patients were instructed to rinse with 15 ml of 0.12% chlorhexidine gluconate mouthwash twice daily (morning and evening) for 1 minute. The treatment duration was 14 days.

5% Morus Nigra Mouthwash

Drug

As an adjunct to SRP, patients were instructed to rinse with 15 ml of a standardized mouthwash containing 5% Morus nigra extract twice daily (morning and evening) for 1 minute. The treatment duration was 14 days.

Scaling and Root Planing (SRP)

Procedure

All patients in the study underwent non-surgical periodontal therapy consisting of full-mouth scaling and root planing (SRP) using both ultrasonic devices and hand instruments.

Primary outcomes

  1. Gingival Index (GI)

    Time frame: Baseline (Day 0), 1 month, and 3 months after periodontal treatment.

    Evaluation of gingival inflammation using a scale of 0 to 3.

  2. Plaque Index (PI)

    Time frame: Baseline (Day 0), 1 month, and 3 months after periodontal treatment.

    Evaluation of plaque accumulation using a scale of 0 to 3.

  3. Probing Depth (PD)

    Time frame: Baseline (Day 0), 1 month, and 3 months after periodontal treatment.

    Measurement of the distance from the gingival margin to the bottom of the periodontal pocket.

  4. Clinical Attachment Level (CAL)

    Time frame: Baseline (Day 0), 1 month, and 3 months after periodontal treatment.

    Measurement of the distance from the cementoenamel junction to the bottom of the periodontal pocket.

  5. Bleeding on Probing (BOP)

    Time frame: Baseline (Day 0), 1 month, and 3 months after periodontal treatment.

    Evaluation of the presence or absence of bleeding within 30 seconds after probing. The result is recorded as a percentage of sites that bleed.

Secondary outcomes

  1. Change in Gingival Crevicular Fluid (GCF) Inflammatory Markers

    Time frame: At baseline, 1 month, and 3 months after treatment

    Analysis of TNF-a, IL-1b, and IL-10 levels in the gingival crevicular fluid using ELISA (or specific biochemical assay) to evaluate the local inflammatory response

  2. Change in Subgingival Plaque Microbiological Profile

    Time frame: At baseline, 1 month, and 3 months after treatment

    Evaluation of subgingival plaque samples to determine the levels of red complex bacteria and Fusobacterium nucleatum using PCR (Polymerase Chain Reaction) analysis

Sponsors and collaborators

Lead sponsor

Inonu University

Other

Registry information

Official study title

Comparison of the Efficacy of Chlorhexidine and Morus Nigra Mouthwashes in the Treatment of Periodontal Diseases

Important dates

Study start
2025
Primary completion
2026
Study completion
2026
First posted
Apr 20, 2026
Registry last updated
Apr 20, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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