Efficacy and Safety of Tirofiban for Patients With BAD (BRANT)

BRANT study is a multicenter, randomized, open label, blinded endpoint, Parallel controlled trial with the primary null hypothesis that, in patients with acute BAD-related stroke, there is no difference in the proportion of excellent outcome in those treated with intravenous Tirofiban compared with those treated with standard antiplatelet therapy based on guideline when subjects are randomized within 48 hours of stroke onset.

The primary objective is to determine whether intravenous tirofiban (a loading dose of 0.4ug/kg/min*30min followed by a maintenance dose of 0.1ug/kg/min*47.5h) is effective in increasing the proportion of excellent functional outcome (mR: 0-1) at 90 days, when initiated within 48 hours of onset. The active comparator is standard antiplatelet therapy based on guideline [ie, 1) aspirin 150-300 mg qd, OR 2) aspirin 100 mg qd plus clopidogrel 75 mg qd] for 48 hours.

Patients with acute BAD-related stroke between 18 and 75 years old, who can be randomized within 48 hours of onset, and meet the BAD Diagnostic Imaging Criteria, will be enrolled. All patients will conduct MRI before randomization.

Subjects will be randomized 1:1 (Tirofiban: Standard antiplatelet therapy). The subjects' eligibility will be assessed by site investigator prior to accessing the Randomization Module, which is generated via the dynamic block randomization method. Only certified and trained personnel can access the randomization website, who will get the information of treatment (ie, Tirofiban or standard antiplatelet therapy) after the subject has be determined eligible.

The treatment period is 48 hours for both study groups. A total of 516 eligible patients will be enrolled. Each participant will be followed for 90 days from randomization. The primary outcome will be assessed by well-trained senior neurologists blinded to the treatment. All the clinical and safety events will be re-examined by the Clinical Event Committee (CEC), who are blinded during all procedures.

Inclusion criteria

• Age: 18-75 years old
• Acute ischemic stroke
• Time from onset to randomization ≤48h; if onset time is unknown, time from last known well to randomization ≤48h
• Meet the following BAD Diagnostic Imaging Criteria

4.1. DWI infarcts: single (isolated) deep (subcortical) infarcts;

4.2. The culprit arteries are either Lenticulostriate artery (LSA) or Paramedian pontine artery (PPA), and the infarct lesion on DWI conforms to one of the following characteristics (A/B): A. LSA: 1) "Comma-like" infarct lesions with "Fan-shaped" extension from bottom to top in the coronal plane; or 2) ≥ 3 layers (layer thickness 5-7 mm) on axial DWI brain images; B. PPA: The infarct lesion extends from the deep pons to the ventral pons on the axial DWI brain images;

4.3. No more than 50% stenosis on the parent artery of the criminal artery (i.e. corresponding basilar or middle cerebral artery) (Confirmed by magnetic resonance angiography [MRA] or computed tomography angiography [CTA] or digital substraction angiography [DSA]).

• Singed informed consent by the patient or legally authorized representatives.

Exclusion criteria

• Transient ischemic attack (TIA)
• Intracranial hemorrhagic diseases, vascular malformations, aneurysms, brain abscesses, malignant space-occupying lesions, or other non-ischemic intracranial lesions detected by baseline CT/MRI, or MRA/CTA/DSA;
• Presence of ≥50% stenosis in extracranial artery in tandem relationship ipsilateral to the lesion;
• Cardiogenic embolism: atrial fibrillation, myocardial infarction, heart valve disease, dilated cardiomyopathy, infective endocarditis, atrioventricular block disease, heart rate less than 50 beats per minute
• Have received or plan to receive endovascular therapy or thrombolysis after onset;
• Stroke of other clear causes, e.g., moyamoya disease, arterial dissection, vasculitis, etc.
• modified Rankin Scale ≥2 before onset
• Use of tirofiban within 1 week before or after onset
• Low platelets (<100×10^9 /L), or Prothrombin time >1.3 times of the upper normal limit, or INR >1.5, or other systemic hemorrhagic tendencies such as hematologic disorders
• Elevation of ALT or AST more than 1.5 times the upper normal limit;
• Glomerular filtration rate <60 ml/min/1.73m^2
• Known malignant tumors
• History of trauma or major surgical intervention within 6 weeks prior to onset
• History of intracranial hemorrhage
• Active or recent history(within 30 days prior to onset) of clinical bleeding (e.g., gastrointestinal bleeding)
• Malignant hypertension (systolic blood pressure >200 mmHg, or diastolic blood pressure >120 mmHg)
• Life expectancy ≤ 6 months
• Contraindications of 3 T MRI examination
• Pregnant or lactating women
• Have participated in another clinical trial within 3 months prior to the date of informed consent, or are participating in another clinical trial.