NCT Number: NCT06727500
The randomized controlled trial will compare the effects of a High-intensity interval training (HIIT) protocol versus a HIIT plus plyometric training in the muscle-tendon structure function and metabolism of the pediatric population with obesity at different biological maturational stages (pre-to-age peak velocity [APHV] and post-APHV). Both groups perform baseline evaluations of the main and secondary outcomes and receive the intervention for twelve weeks, three times per week. Once the interventions are completed, the participants undergo the same evaluations they performed at baseline.
Interested in participating?
Request Info7 year–17 year
All sexes
Interventional
Not applicable
Santiago, Chile
Location status: Recruiting
Mauricio Inostroza-Mondaca, MSc.
CONTACT
Rodrigo Ramirez-Campillo, MSc. PhD.
CONTACT
The prevalence of pediatric obesity increased 4.2-fold between 1976 and 2022, and it's expected to continue increasing toward 2030. Pediatric obesity is a public health problem due to its multiple metabolic comorbidities. Moreover, body weight and fat infiltration due to pediatric obesity negatively affect muscle-tendon structure, function, and metabolism, negatively impacting physical activity levels, active play, functional performance (e.g., jumping capacity), and quality of life. As a solution, HIIT protocols could decrease body weight and fat percentage and increase muscle oxidative capacity. The addition of plyometric training to HIIT protocols could generate the same effects as the HIIT protocol alone, with an additional enhancement in functional performance. However, the influence of biological maturation stages in the adaptations to training in pediatric population with obesity has been scarcely reported. Thus, throughout different biological maturation stages, such as pre-to-age peak velocity (APHV) (early stage of biological maturation) to post-APHV (final stage of biological maturation stage), an increase in testosterone, growth factors, and glycolytic metabolism capacity occurs. Therefore, the differences in the anabolic milieu and glycolytic metabolism between the pre-APHV and post-APHV could produce a differential capacity of training stimuli to modify the muscle-tendon, structure, function, and metabolism in these pediatric population with obesity. Therefore, this study aims to determine the effects of HIIT and concurrent HIIT and plyometric training in the muscle-tendon structure, function, and metabolism of the pediatric population with obesity in different maturation stages.
The study will be a parallel-group randomized controlled trial that compares the effects of HIIT versus HIIT plus plyometric training on muscle-tendon structure, function, and metabolism in the pediatric population pre-APHV and post-APHV with obesity in a public health setting. The primary outcome will be the Achilles tendon mechanical properties. We estimate a sample size of 10 participants per group and 40 in total.
The investigators aim to demonstrate the beneficial effects of HIIT plus plyometric training on the muscle-tendon structure and metabolism of the pediatric obesity population. Moreover, the researchers expected a difference in the adaptations provided by the training protocol between the pre-APHV and post-APHV groups.
Healthy volunteers accepted: No
Only the study team can determine whether someone qualifies for participation.
Inclusion criteria
Exclusion criteria
The HIIT plus plyometric training will perform two weeks of exercise adaptation involving 25 minutes of MICT at 65% of maximal heart rate (HRmax) and 5 minutes of jumps in place at 5 - 6 of the EPinfant scale of Perceived exertion. The HIIT plus plyometric training will perform 15 minutes of HIIT and 5 minutes of jumps each session. The HIIT phase will be performed in a work recovery intensity at 85 and 50% of the HRmax. The work phase of jumps will be performed "all out," and the recovery phase will be performed at 5 - 6 of the EPinfant scale of perceived exertion. The work: recovery bouts will be progressed from 1:4 (15 seconds: 60 seconds) at weeks 3-6, to 1:3 (15 seconds: 45 seconds) at weeks 7-9, and to 1:3 (15 seconds: 30 seconds) at weeks 10-12. In all sessions, the rate of perceived exertion, heart rate, and exercise planning compliance will be measured.
The HIIT group will perform two weeks of exercise adaptation involving 30 minutes of moderate-intensity continuous training (MICT) at 65% of the maximal heart rate (HRmax) reached in the maximal oxygen consumption test. The HIIT group will perform 20 minutes of HIIT each session, with a work: recovery intensity at 85% and 50% of HRmax, respectively. The work: recovery bouts will be progressed from 1:4 (15 seconds:60 seconds) at weeks 3-6, to 1:3 (15 seconds:45 seconds) at weeks 7-9, and to 1:2 (15 seconds: 30 seconds) at weeks 10-12. In all sessions, the rate of perceived exertion, heart rate, and exercise planning compliance will be measured.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The stiffness will be estimated as the slope of the linear tendon elongation-force relationship above 50% of each participant's maximum attained force.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The Achilles tendon young modulus will be estimated as the slope of the linear tendon stress-strain relationship above 50% of each participant's maximum attained force.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The Achilles tendon force of the Achilles tendon will be calculated as the plantar flexors torque divided by the Achilles tendon moment arm plus the dorsiflexor contribution.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
Tendon elongation will be measured as the Achilles tendon myotendinous junction displacement (ArtUS, Telemed, Vilnius, Lithuania) from rest in the MIVC trials. The tendon lengthening will be normalized by the Achilles tendon moment arm.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The tendon stress will be calculated as the ratio between the mean maximum attained force and the smallest cross-sectional area measured for each participant.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The tendon strain will be calculated as the tendon elongation associated with the peak tendon force relative to its resting length.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
To determine the gastrocnemius medialis thickness, the children will be measured in a prone position with the knee fully extended and the ankle at 90°. A B-mode ultrasound (ArtUS, Telemed, Vilnius, Lithuania) will take images at 20%, 30%, and 40% of the tibial length, defined as the distance from the popliteal crease to the midpoint of the lateral malleolus. Muscle thickness will be defined as the perpendicular distance between superficial and deep aponeurosis.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
To determine the gastrocnemius medialis pennation angle, the children will be measured in a prone position with the knee fully extended and the ankle at 90°. A B-mode ultrasound (ArtUS, Telemed, Vilnius, Lithuania) will take images at 20%, 30%, and 40% of the tibial length, defined as the distance from the popliteal crease to the midpoint of the lateral malleolus. Pennation angle will be defined as the angle between the deep aponeurosis and the echoes of the interspace between the fibers bundles.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
To determine the gastrocnemius medialis fascicle length, the children will be measured in a prone position with the knee fully extended and the ankle at 90°. A B-mode ultrasound (ArtUS, Telemed, Vilnius, Lithuania) will take images at 20%, 30%, and 40% of the tibial length, defined as the distance from the popliteal crease to the midpoint of the lateral malleolus. The fascicle length will be derived through the means of trigonometry using the values of muscle thickness and pennation angle.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
To determine the gastrocnemius medialis echo-intensity, the children will be measured in a prone position with the knee fully extended and the ankle at 90°. A B-mode ultrasound (ArtUS, Telemed, Vilnius, Lithuania) will take images at 20%, 30%, and 40% of the tibial length, defined as the distance from the popliteal crease to the midpoint of the lateral malleolus. The echo intensity will be measured as the mean of pixel intensity in a region of interest.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The MVIC will be assessed in a prone position with the knee fully extended with the ankle at 90° in a dynamometer (Isoforce, TUR, Germany). Participants will perform three submaximal contractions of the plantar flexors at 50%, 70%, and 90% of the MIVC and three attempts to reach the MIVC separated by 1 minute of rest. The participants will perform three attempts to reach the MIVC under the instruction to perform "as fast as possible".
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The participants will perform three attempts to reach the MIVC under the instruction to perform "as fast as possible." with this consideration, we will calculate the rate of force development (RFD) offline. The RFD will calculate the change in force per time interval at 50, 100, 200, and 400 ms using a custom-written program.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
Participants will perform a treadmill (HP cosmos, LE 200 CE model, Germany) maximal oxygen consumption test (Cosmed, Quark CPET, Italy) until exhaustion. The VO2 peak will be taken at the highest 10 seconds average until exhaustion, and the gas exchange threshold (GET) will be calculated with the V-slope method.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
To determine the VO2 kinetics, the participants will complete four "step" tests. The "step" test will consist of a transition for the rest to running at 90% of the GET for 6 minutes, to perform a second "step" test of the heart rate, VO2, dioxide carbon production (VCO2), and Ventilation minute (VE) will return to baseline levels. Throughout the 6 minutes running at 90% GET, phase II of the VO2 kinetics will be plotted against time.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
During the four "step" test, a Moxy monitor (Moxy, fortiori LLC, Minnesota, USA) will be ubicated in the gastrocnemius medialis to measure the total hemoglobin and muscle oxygen saturation.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
Venous blood samples will be collected after 8 to 12 hours of fasting. Data from the venous samples will be used to calculate the lipid profile (triglycerides, total cholesterol, LDL cholesterol, VLDL cholesterol and HDL cholesterol).
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
Venous blood samples will be collected after 8 to 12 hours of fasting. Data from the venous samples will be used to calculate the glycemic control (glycemia, insulin). The HOMA-index will calculated by dividing fasting insulin (mU/L) by glycemia (mg/dl) / 405.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
Venous blood samples will be collected after 8 to 12 hours of fasting. Data from the venous samples will be used to calculate the low-grade inflammation state by reactive C protein and IL-6.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
A physician will measure blood pressure on an outpatient basis using equipment appropriate for the patient arm length and age. The obtained data will be classified by sex, height, and age according to the normative values provided in the clinical guidelines for the diagnosis and management of high blood pressure in the pediatric population.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The standing broad jump will be assessed following the alpha fitness protocol.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The squat jump will be assessed with a contact platform
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The countermovement jump will be assessed with a contact platform
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
A Bioimpedance device (InBody770 model, South Korea) will be used for the body composition analysis. Participants will be asked to avoid exercise, caffeine for ≥ 24 hours, and food or drinks for ≥4 hours before testing, with the exception of water, which could be consumed up to 45 minutes before testing.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
A Bioimpedance device (InBody770 model, South Korea) will be used for the body composition analysis. Participants will be asked to avoid exercise, caffeine for ≥ 24 hours, and food or drinks for ≥4 hours before testing, with the exception of water, which could be consumed up to 45 minutes before testing.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
A Bioimpedance device (InBody770 model, South Korea) will be used for the body composition analysis. Participants will be asked to avoid exercise, caffeine for ≥ 24 hours, and food or drinks for ≥4 hours before testing, with the exception of water, which could be consumed up to 45 minutes before testing.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
A Bioimpedance device (InBody770 model, South Korea) will be used for the body composition analysis. Participants will be asked to avoid exercise, caffeine for ≥ 24 hours, and food or drinks for ≥4 hours before testing, with the exception of water, which could be consumed up to 45 minutes before testing.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
Body weight were obtained using a SECA® beam balance
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
Body height were obtained using a SECA® stadiometer.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
Based on the weight and height measurements, BMI and BMI-z scores were calculated.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
Waist circumference was measured using a non-stretchable measuring tape.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
Using the height and waist circumference, the waist-to-height ratio (WHtR) was calculated.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The Kidscreen-27 questionnaire will be used to assess the quality of life of the pediatric population with obesity. The questionnaire consists of five dimensions, allowing detailed profile information for physical well-being, psychological well-being, autonomy, parent relations, social support, peers, school, and environment. The questionnaire was validated in the Chilean pediatric population.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The Spanish Perceived Physical Literacy Inventory will be used to assess the physical literacy of children. The questionnaire consists of three items (sense of self and self-confidence, self-expression, communication with others, and knowledge and understanding) divided into nine questions. It was validated in the Spanish language.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The Physical Activity Enjoyment Scale will be used to assess the enjoyment of the two different training programs. The questionnaire consists of 16 Likert-type questions, which were validated in the Spanish language and present a Cronbach's Alpha coefficient of 0.85, informing an internal consistency of the scale.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
For the male pediatric population, the formula to determine the years for APHV will be: -7.99994 + (0.0036124) x (chronological age x height), and for the female pediatric population, it will be: -7.709133 + (0.0042232) x (chronological age x height). The pediatric population with a maturity offset of less than -1 will be determined as pre-APHV and greater than +1 as post-APHV.
Time frame: Previous (T0) and posterior to twelve weeks of training (T12).
The questionnaire of the "Instituto de Nutrición y Tecnología de los Alimentos" will be used to assess the physical activity levels. The questionnaire was validated in the Chilean population and consists of five items: hours spent lying down, hours spent in seated activities, number of blocks walked, daily hours spent in outdoor recreational games, and weekly hours of scheduled exercises or sports. Each category will be scored from 0 to 2, so the total score ranges from 0 to 10. The score of 5 presents a specificity value of 0.89 to determine the inactive pediatric population
Universidad Nacional Andres Bello
Other
Effects of HIIT and Concurrent HITT/Plyometric Training on Muscle-tendon Structure, Function and Metabolism in Pediatric Population With Obesity at Different Biological Maturation States (HIIT-PRO Kids): A Study Protocol of Randomized Controlled Trial
Acronym: HIIT-PRO-kids
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