Skip to main content
OpenTrials
Completed

NCT Number: NCT04596553

Effects of Essential and Non-Essential Amino Acids on Post-Exercise Muscle Collagen Synthesis in Young Men

The purpose of this study is to assess which amino acids are affecting acute muscle collagen synthesis in response to supplementation and exercise.

Completed

Looking for future studies?

Notify Me

Key information

Conditions

Skeletal Muscle Amino Acids Metabolism Behavior Dietary Supplements Exercise Extracellular Matrix Motor Activity

Age range

18 year–35 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Chan Gunn Pavilion

Vancouver, British Columbia, V6T 1Z3, Canada

About this study

This is a double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid). The study will involve 3 visits: a baseline testing/familiarization session, and 2 exercise trials which will include muscle biopsies, blood draws, and the infusion of a tracer isotope in order to measure muscle protein synthesis.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Can fluently read and write in English
  • Male
  • 18 - 35 years

Exclusion criteria

  • Diabetes, cardiovascular disease, or current pharmaceutical therapy
  • Medical conditions impacting ability to undertake strenuous physical activity
  • Lower body resistance training in the previous 6 months
  • BMI > 30
  • Currently smoking with cigarettes
  • Allergy to any study supplements
  • Currently taking any protein, amino acid, or collagen supplement
  • Participate in greater than 2 hours of weekly vigorous exercise

Treatment and study plan

Essential Amino Acid

Dietary Supplement

Immediately following the exercise intervention participants will consume their assigned essential amino acid supplement

Collagen Peptide

Dietary Supplement

Immediately following the exercise intervention participants will consume their assigned collagen peptide supplement

Maltodextrin placebo

Dietary Supplement

Immediately following the exercise intervention participants will consume their assigned maltodextrin supplement

Primary outcomes

  1. Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis

    Time frame: 0 - 4 hours post-exercise

    Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.

  2. Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis

    Time frame: 0 - 2 hours post-exercise

    Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.

  3. Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis

    Time frame: 2 - 4 hours post-exercise

    Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.

  4. Fractional synthetic rate (change over time) Muscle Collagen Synthesis

    Time frame: 0 - 4 hours post-exercise

    Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.

  5. Fractional synthetic rate (change over time) Muscle Collagen Synthesis

    Time frame: 0 - 2 hours post-exercise

    Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.

  6. Fractional synthetic rate (change over time) Muscle Collagen Synthesis

    Time frame: 2 - 4 hours post-exercise

    Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.

  7. Pro-Collagen Type I N-Propeptide (PINP)

    Time frame: 1 hour prior to exercise

    Osteoblastic marker of bone collagen synthesis

  8. Pro-Collagen Type I N-Propeptide (PINP)

    Time frame: 4 hours post exercise

    Osteoblastic marker of bone collagen synthesis

Secondary outcomes

  1. Plasma Amino Acids Response to Supplementation

    Time frame: 0 - 4 hours post-exercise

    Amino acid plasma concentrations

  2. Muscle mechanistic target of rapamycin Pathway Activation

    Time frame: 0 - 4 hours post-exercise

    Measuring the fold change in enzymes associated with the anabolic signalling of the mTOR pathway (e.g. phosphoinositide 3-kinases (PI3k), protein kinase B (Akt))

  3. PCR Gene Expression of Muscle Collagen Genes (Types I, III, IV)

    Time frame: 0 - 4 hours post-exercise

    Measuring the fold change when the collagen genes of interest become detectable in the PCR analysis

  4. Pro-Collagen Type III N-Propeptide (PIIINP)

    Time frame: 1 hour prior to exercise

    Marker of collagen synthesis

  5. Pro-Collagen Type III N-Propeptide (PIIINP)

    Time frame: 4 hours post exercise

    Marker of collagen synthesis

Sponsors and collaborators

Lead sponsor

University of British Columbia

Other

Registry information

Official study title

The Effects of Essential and Non-Essential Amino Acids on Post-Exercise Muscle Collagen Synthesis in Young Men: a Randomized Controlled Trial

Acronym: PECS

Important dates

Study start
2021
Primary completion
2022
Study completion
2022
First posted
Oct 22, 2020
Registry last updated
Jun 13, 2023

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Related clinical trials

Published trials that share one or more normalized conditions with this study.