Skip to main content
OpenTrials
Completed

NCT Number: NCT07075497

Effects of Combining PNF With NMES in Young Football Players

Hamstring muscle injuries are common in sports like football and often lead to extended periods of inactivity. This study aims to compare the effects of two different stretching programs on hamstring flexibility, with a secondary focus on jumping performance in young football players. One group will perform a proprioceptive neuromuscular facilitation (PNF) stretching protocol combined with neuromuscular electrical stimulation (NMES), while the other group will follow the same PNF protocol with a placebo NMES.

Completed

Looking for future studies?

Notify Me

Key information

Age range

10 year–18 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

University Ceu Cardenal Herrera

Alfara del Patriarca, Valencia, 46113, Spain

About this study

Hamstring strains are increasingly common in sports, particularly in football, where they are among the most frequent injuries. Stretching techniques such as proprioceptive neuromuscular facilitation (PNF) are commonly used to improve muscle flexibility and reduce injury risk. A specific variant, contract-relax PNF (crPNF), involves alternating passive stretching with voluntary muscle contractions. Recent approaches suggest that applying neuromuscular electrical stimulation (NMES) during the contraction phase of crPNF may further enhance its effectiveness.

This study aims to compare the short-term effects of two active stretching protocols on hamstring flexibility in young male football players. One group will perform crPNF with sham NMES, while the other will perform crPNF with NMES during the contraction phase. A secondary objective is to assess whether either protocol affects vertical jump performance, a key ability in football.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Active football players who train at least 3 days a week.
  • Ages between 10 and 18 years.

Exclusion criteria

  • Are currently engaged in a structured hamstring-stretching programme.
  • Present with acute low back pain.
  • Have sustained a hamstring injury within the past 6 months.
  • Have undergone spinal or abdominal surgery within the past 6 months.

Treatment and study plan

Neuromuscular electrical stimulation (NMES)

Other

NMES is applied during each 10-second isometric hamstring contraction using an Enraf Nonius TensMed S82 stimulator delivering biphasic, symmetric rectangular pulses at 50 Hz with a 250 µs pulse width. Two 5 × 5 cm self-adhesive electrodes are placed on the hamstrings-one approximately two finger-breadths below the gluteal fold and the other over the distal third of the muscle belly. Stimulation intensity is individually titrated to each participant's maximal motor threshold-eliciting a strong, yet painless, contraction-and the exact milliamperage is recorded for each leg. Elastic straps over the pelvis and distal thigh maintain pelvic anteversion and knee extension. During each session, one researcher secures limb positioning while a second adjusts and logs NMES intensity and cues the timing of the 10 s contraction and ensuing 30 s passive stretch.

Sham Neuromuscular electrical stimulation (NMES)

Other

Sham NMES is delivered during each 10 s isometric hamstring contraction using an Enraf Nonius TensMed S82 stimulator with biphasic, symmetric rectangular pulses (50 Hz, 250 µs pulse width). Two 5 × 5 cm self-adhesive electrodes are placed on the hamstrings-one two finger-breadths below the gluteal fold and one over the distal third of the muscle belly. Intensity is individually set to the sensory threshold, defined as the minimal current producing a slight tingling without any visible muscle response; this milliamperage is recorded for each leg. Elastic straps over the pelvis and distal thigh maintain pelvic anteversion and full knee extension. During each session, one researcher secures limb positioning while a second adjusts and logs the NMES intensity and cues the 10 s contraction timing.

Contract-Relax PNF (crPNF)

Other

The crPNF Group engages in an isolated contract-relax proprioceptive neuromuscular facilitation (crPNF) stretching protocol. Participants are positioned in a long sitting posture with maximum knee extension until they feel a moderate-strong stretch sensation, without pain. Each stretch lasts for 30 seconds, followed by a 10-second maximal voluntary isometric contraction of the hamstrings. Participants complete three cycles of stretch and contraction. One researcher maintains the stretch position while a second researcher controls the timing of the stretching and contractions.

Primary outcomes

  1. The Popliteal Angle Test

    Time frame: Before and inmediately after the intervention

    The Popliteal Angle Test is validated for measuring hamstring extensibility. Participants are placed in the supine position. A researcher holds the hip at a 90° flexion and passively extends the knee until the participant feels a strong stretch without pain. The examiner records the knee extension degrees at that moment using an inclinometer. The test is repeated three times, and the average is obtained. A full knee extension corresponds to a value of 0 degrees, and a higher number of degrees indicates greater hamstring shortening.

  2. The Sit & Reach Test

    Time frame: Before and inmediately after the intervention

    The Sit & Reach Test has high intra-examiner reliability and is validated for measuring hamstring extensibility. For its execution, participants are placed in a long sitting position with the soles of their feet against the base of the measurement box. Keeping their knees extended, they reach for the maximum possible distance in the box with their fingers. The test is repeated three times, and the average is obtained. Values are recorded in centimeters, where a greater distance indicates a higher degree of hamstring extensibility.

Secondary outcomes

  1. The Counter Movement Jump

    Time frame: Before and inmediately after the intervention

    The Counter Movement Jump is validated and standardized in adolescents for measuring jumping ability. The instructions by Petrigna et al. are followed to perform the jump. The My Jump App is used, which has high reliability and validity for measuring vertical jump height in centimeters. A greater height indicates a greater jumping ability.

Sponsors and collaborators

Lead sponsor

Cardenal Herrera University

Other

Registry information

Official study title

Short Term Effects of Proprioceptive Neuromuscular Facilitation Combined With Neuromuscular Electrical Stimulation in Young Football Players: a Randomized Clinical Trial

Acronym: PNF+NMES

Important dates

Study start
2025
Primary completion
2025
Study completion
2025
First posted
Jul 20, 2025
Registry last updated
Nov 17, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Published trials that share one or more normalized conditions with this study.