Effect of Total Sleep Deprivation on Vascular Function

Inclusion criteria

• Males and females
• 20 to 30 and 50 to 60 years of age
• Females will be eumenorrheic at enrollment or postmenopausal for at least 1 year
• No major clinical disease (e.g., diabetes, cardiovascular, liver or chronic kidney disease) to minimize confounding of vascular measures
• Participants will be sedentary (≤3x per week of ≤30 minutes of aerobic exercise/session) or trained (≥5x per week of moderate/vigorous aerobic exercise training)

Exclusion criteria

• Age <20 or 31 to 49 or >60 years
• Body mass index ≥30 kg/m^2 because obesity may affect vascular function
• Use of medication that may affect vascular measures
• Hormone replacement therapy or hormonal contraceptives within past year
• Use of tobacco products (chewing tobacco, traditional or e-cigarettes) because they may influence vascular function
• Being perimenopausal, pregnant or lactating because may influence vascular function
• Being a shift worker because habitual sleep deprivation and altered circadian rhythm may influence vascular function.
• Not having an "intermediate" chronotype based on the Morningness-Eveningness Questionnaire (MEQ; score <31 or >69) because this would influence the effect of overnight sleep deprivation on vascular function.
• Sleep complaints based on the Pittsburgh Sleep Quality Index (PSQI; score >5) because this would influence the effect of overnight sleep deprivation on vascular function.