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OpenTrials
Completed

NCT Number: NCT03470363

Effect of Spinal or Sevoflurane Anesthesia on Neutrophil Activation During Knee Surgery With Pneumatic Tourniquet

Aim of study : Assessement of neutrophil activation during ischemia reperfusion associated with tourniquet use for total knee arthroplasty. This activation was compared in a group of patient scheduled for spinal anesthesia and for sevoflurane anesthesia

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Key information

Conditions

Knee Surgery With Tourniquet

Age range

18 year and older

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

About this study

Aim of study : Assessement of neutrophil activation during ischemia reperfusion associated with tourniquet use for total knee arthroplasty. This activation was compared in a group of patient scheduled for spinal anesthesia and for sevoflurane anesthesia This activation was assessed by plasma myeloperoxidase and elastase measurements in four consecutive blood samples during and after surgery (before surgical incision and 1, 3, 24 hours following tourniquet release.

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Total knee arthroplasty under spinal or sevoflurane anesthesia

Exclusion criteria

  • American Society of Anesthesiologists class 3 and 4
  • vascular disease of the lower limbs
  • cardiac or respiratory disease
  • immune disorder

Treatment and study plan

Sevoflurane Volatile Liquid

Drug

Bupivacaine / SUFentanil

Drug

Primary outcomes

  1. Changes in plasmatic concentration of myeloperoxidase and elastase

    Time frame: Before surgical incision and one hour after tourniquet release

    Biological measurement of changes in plasmatic concentration of myeloperoxidase and elastase from preoperative values to one hour after tourniquet release.

  2. Changes in plasmatic concentration of myeloperoxidase and elastase

    Time frame: Before surgical incision then 3 and 24 hours after tourniquet release

    Biological measurement of changes in plasmatic concentration of myeloperoxidase and elastase from preoperative values to 3 and 24 hours after tourniquet release.

Secondary outcomes

  1. Changes in plasmatic concentration of endothelial biomarkers

    Time frame: Before surgical incision and one hour after tourniquet release

    Biological measurement of changes in plasmatic concentration of syndecan-1, soluble thrombomodulin, soluble E-selectin and VEGF from preoperative values to one hour after tourniquet release.

  2. Changes in plasmatic concentration of endothelial biomarkers

    Time frame: Before surgical incision then 3 and 24 hours after tourniquet release

    Biological measurement of changes in plasmatic concentration of syndecan-1, soluble thrombomodulin, soluble E-selectin and VEGF from preoperative values to 3 and 24 hours after tourniquet release.

Sponsors and collaborators

Lead sponsor

University of Liege

Other

Registry information

Official study title

Effect of Spinal or Sevoflurane Anesthesia on Neutrophil Activation After Tourniquet Induced Ischemia-reperfusion in Knee Surgery

Important dates

Study start
2016
Primary completion
2018
Study completion
2018
First posted
Mar 19, 2018
Registry last updated
Mar 26, 2020

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.