Effect of Mouthwashes on Oral Wound Healing and Microbiome

Inclusion criteria

• Male or female volunteers 18-45 years of age.
• Good general health.
• Must sign informed consent form.
• Minimum of 17 natural uncrowned teeth (excluding third molars) must be present.

Exclusion criteria

• Oral pathology, chronic disease, or history of allergy to testing product.
• Medical condition which requires pre-medication prior to dental visits/procedures
• Moderate or advanced periodontal disease (gum disease) or periodontal probing depths greater than 4 mm.
• Five (5) or more decayed untreated dental sites at screening (cavities).
• Impaired salivary function.
• Use of medications that can currently affect salivary flow.
• Use of anticonvulsants, antihistamines, antidepressants, sedatives, tranquilizers, antibiotics, antimicrobial, anti-inflammatory medication or daily analgesics within 30 days prior to the start of the study or scheduled to start such intake during the course of the study.
• Ongoing use of medications known to affect the gingival tissues (i.e. calcium channel blockers, phenytoin, cyclosporine)
• Pregnant or nursing women.
• Participation in any other clinical study within 1 week prior to enrollment into this study.
• Use of any tobacco products.
• Subjects who must receive dental treatment during the study dates.
• Presence of an orthodontic appliance that interferes with required clinical assessments.
• History of allergy to common dentifrice ingredients.
• Subjects who wear night guard, denture retainers
• Not willing to abstain from eating crunchy (eg. Granola bars, popcorns, nuts etc) or spicy and hot foods/liquids during the study period;
• Not willing to abstain from drinking citrus fluids, alcohol, and tea during the study period
• Non English-speaking subjects