Effect of Internal and External Micro-needling in Treatment of Stage III Periodontitis

Study Settings and Source of Patients The study will take place in the outpatient clinics of the oral medicine, periodontology, and Diagnosis department at the Faculty of Oral and Dental Surgery at Misr University for Science and Technology. All procedures will be conducted with the approval of the Research Ethics Committee (REC) of the Faculty of Oral and Dental Surgery at Misr University for Science and Technology.

SIX periodontitis stage III patients seeking periodontal therapy will be included in this study. The selection of patients will be done after careful clinical, radiographic, and periodontal examination according to the following inclusion and exclusion criteria.

Methods of measurement of outcome:

Clinical Parameters:

The following parameters will be recorded at baseline, 3 months after initial periodontal therapy and after 6 months.

• Probing depth:

UNC 15 probe will be used to measure the pocket depth from the gingival margin to the base of the pocket.

• Gingival index [GI]:

It is used to assess gingival inflammation .

• Clinical attachment level [CAL]):

Represents the extent of periodontal support that has been lost around a tooth and is measured with the periodontal probe as the distance from the cemento-enamel junction (CEJ) to the base of the pocket .

• plaque index:

The desirability of distinguishing clearly between the soft debris aggregates' severity and location.

• Bleeding on probing POB:

bleeding indices have been devised; some assess bleeding as simply present or absent, whereas others use grading in an attempt to assess severity of bleeding

• Vas index :

A Visual Analogue Scale (VAS) is a pain rating scale 1,2,3,4,5,6,7,8,9 Scores are based on self-reported measures of symptoms that are recorded with a single handwritten mark placed at one point along the length of a 10-cm line that represents a continuum between the two ends of the scale-"no pain" on the left end (0 cm) of the scale and the "worst pain" on the right end of the scale (10 cm)

Biological assessment(Platelet-derived growth factor(PDGF) levels in gingival crevicular fluid Assessment of PDGF in gingival crevicular fluid at baseline,7,14 days

Inclusion criteria

• Male or Female systematic health
• age ≥ 18 years
• Provide informed consent and willingness to cooperate with the study protocol.
• Periodontitis stage III patients:
• Probing depth≥6 mm
• CAL ≥5 mm.
• Radiographic bone loss (Extending to middle and beyond)
• Tooth loss ≥4 teeth.
• Each patient contributed a maximum of two sides.

Exclusion criteria

• smoker
• History of antibiotic in previous three months.
• Pregnant or lactating.
• Treatment with any systemic drug that influences the healing of hard and soft tissues.
• Patients who received periodontal treatment in the last 6 months