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NCT Number: NCT06561971

Effect of Different Centrifuge Values on Saliva High Sensitive C-Reactive Protein (Hs-CRP) Levels in Individuals With Different Smoking Amounts

The purpose of this study; In periodontally healthy individuals, high sensitive c-reactive protein (hs-CRP) levels of non-smokers, smokers of less than 10 cigarettes per day and smokers of more than 10 cigarettes per day were measured at 15 rpm-10 min, 10 centrifuge values, which are frequently used in saliva studies. To determine the levels in saliva samples as a result of rpm-10 min and 10 rpm 6 min centrifugations and to determine the values at which a cytokine of this molecular weight should be centrifuged and to examine the possible correlation between these values and the clinical parameters of the amount of smoking. Materials and Methods: saliva samples were collected from 90 systemically healthy individuals who were non-smokers (N, n=30), light smokers <10 cigarettes per day (L, n=30) and heavy smokers >10 cigarettes per day (H, n=20). samples were taken. Full-mouth clinical periodontal measurements, including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI), and plaque index (PI), were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine hs-CRP levels in biological samples.

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Key information

Conditions

Smoking Behavior

Age range

18 year–30 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Izmir Katip Çelebi University Department of Periodontology

Izmir, Çiğli, 35640, Turkey (Türkiye)

Location status: Recruiting

Location contact

Ece Özer

CONTACT

[email protected]

+905306918625

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Systemically healthy
  • Periodontally clinical healthy
  • At least 20 permanent teeth in the mouth
  • No medication for continuous use
  • Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
  • Not in pregnancy or lactation period.
  • Not received periodontal treatment in the last 6 months
  • For the non-smoker group; person who does not smoke tobacco
  • For the light-smoker group; person who smokes less than 10 cigarettes a day
  • For heavy-smoker group; person who smokes more than 10 cigarettes a day

Exclusion criteria

  • Any oral or systemic disease
  • Regularly using a systemic medication
  • During pregnancy or lactation
  • Received periodontal treatment within the last 6 months.
  • Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
  • Individual with periodontal disease

Treatment and study plan

saliva obtaining

Diagnostic Test

The patient was asked to sit upright and tilt his/her head forward to collect saliva samples. İn this way, unstimulated saliva was allowed to accumulate in the floor of the mouth. The accumulated saliva was collected in a sterile container. It was then transferred to a propylene tube. The tubes were centrifuged and the clear part at the top of the tube was taken with a sterile syringe and transferred to a different propylene tube with 0.5 ml in each tube. Tubes were stored at -80ºC until the day of analysis.

Primary outcomes

  1. The total amount of hs-CRP in saliva

    Time frame: 24 hours after taking the clinical measurements at the first visit

Secondary outcomes

  1. Determining the ideal centrifugal value range of hs-CRP saliva level

    Time frame: Within 1 week after receiving the biochemical analysis results

Study contacts

Contact information is provided by the study sponsor or research team.

Ece Özer

CONTACT

[email protected]

+90 5306958625

Mehmet Sağlam

CONTACT

Sponsors and collaborators

Lead sponsor

Izmir Katip Celebi University

Other

Registry information

Official study title

Evaluating the Effect of Smoking Amount on Determining Salivary High Sensitive C-Reactive Protein(Hs-CRP) Values by Centrifuging the Saliva of the Same Individuals at Different Rpm and Time.

Important dates

Study start
2024
Primary completion
2024
Study completion
2025
First posted
Aug 20, 2024
Registry last updated
Dec 20, 2024

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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