Effect of Cannabis Consumption on Sperm Nuclear Quality in Infertile Men

Inclusion criteria

• Male Patient,
• Patient age ≥ 18 years,
• Infertile patient with isolated teratozoospermia or associated with asthenozoospermia and / or oligozoospermia and / or necrozoospermia, defined according to WHO recommendations (WHO guidelines, 2010) and the David amended classification (Auger et al, 2001) for teratozoospermia
• Patient with normal constitutional karyotype (46, XY).
• Smoking tobacco,
• Drinking ≤ 20 g (2 units) / day,
• Patient exposed : Cannabis user for over 3 months and consuming at least weekly (≥ 1 / week) [questionnaire and positive blood detection of Delta-9-Tetrahydrocannabinol (THC) and / or its derivatives (11-hydroxy-THC and 11-nor-9-carboxy-THC)].
• Unexposed : No cannabis user (questionnaire and negative blood detection of Delta-9-THC and its derivatives) matched for age (+/- 2.5 years) with exposed patients included,

Exclusion criteria

• Patient age > 60 years
• Patient with azoospermia
• Patient previously exposed to gonadotoxic treatment (chemotherapy, radiotherapy, androgen therapy and other gonadotoxic treatments),
• Patient with professional toxic exposure,
• Patient consuming other recreational drugs,
• Patient with severely impaired sperm parameters and sperm counts <1 million,