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Completed

NCT Number: NCT04156113

Effect of an Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism

This study aims to determine the effect of an acute maximal exercise on serum uncoupling protein-1, irisin, interleukin 6 levels, and on blood lipid and lipoprotein concentrations. This study also evaluates the role of uncoupling protein-1-3826 A/G polymorphism on this eventual effect.

Therefore, this study hypothesizes that:

H01: Sedentary people and athletes' serum uncoupling protein 1, irisin, and interleukine 6 levels are different before and after an acute maximal exercise.

H02: Sedentary people and athletes' basal serum uncoupling protein 1 levels are associated with uncoupling protein 1-3826 A/G polymorphism.

H03: Uncoupling protein 1-3826 A/G polymorphism has a modifying role in the effect of maximal exercise on serum uncoupling protein 1 levels.

H04: Sedentary people and athletes' basal serum lipid and lipoprotein levels are associated with uncoupling protein 1-3826 A/G polymorphism.

H05: Uncoupling protein 1-3826 A / G polymorphism has a modifying role in the effect of maximal exercise on serum lipid and lipoprotein levels.

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Key information

Conditions

Uncoupling Protein-1 Irisin Polymorphism, Restriction Fragment Length

Age range

18 year–35 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Ege University Faculty of Sport Sciences

Izmir, Turkey (Türkiye)

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Athletes group:
  • Must be basketball, or volleyball, or handball players.
  • Must be subjects who have been training regularly for at least 3 months.
  • Sedentary group:
  • Must be subjects who have not been training regularly for at least 3 months.
  • For both groups:
  • Healthy.
  • Non obese (body mass index < 30).
  • Aged between 18 and 35 years.

Exclusion criteria

  • For both groups:
  • Being regular consumer of cigarettes, alcohol, drugs and antioxidant substances.
  • Being obese (body mass index > 30).
  • Having an acute or chronic injury which is limiting physical performance.
  • Having a disease (anemia, infection...) detected from the biochemical analysis of the blood samples.
  • Being injured or sick during the study.

Treatment and study plan

Yo-Yo intermittent recovery test (level 1)

Other

The Yo-Yo intermittent recovery test (level 1) will be administered once.

Primary outcomes

  1. Change from the baseline serum uncoupling protein 1 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Uncoupling protein 1 analyzes were performed in the laboratory.

  2. Change from the baseline serum irisin level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Irisin analyzes were performed in the laboratory.

  3. Uncoupling protein-1-3826 A/G polymorphism determination

    Time frame: 15 minutes before the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1). Polymorphism was determined in the laboratory.

Secondary outcomes

  1. Change from the baseline serum interleukin-6 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Interleukine-6 analyzes were performed in the laboratory.

  2. Change from the baseline serum nitric oxide level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Nitric oxide analyzes were performed in the laboratory.

Other outcomes

  1. Change from the baseline serum C reactive protein level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. C reactive protein analyzes were performed in the laboratory.

  2. Change from the baseline serum creatine kinase level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Creatine kinase analyzes were performed in the laboratory.

  3. Change from the baseline serum alanine aminotransferase level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Alanine aminotransferase analyzes were performed in the laboratory.

  4. Change from the baseline serum aspartate aminotransferase level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Aspartate aminotransferase analyzes were performed from serum samples in the Medical Biochemistry Laboratory of the Ege University Faculty of Medicine.

  5. Change from the baseline hemogram test at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Hemogram test analyzes were performed in the laboratory.

  6. Change from the baseline at serum glucose level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Glucose analyzes were performed in the laboratory.

  7. Change from the baseline serum urea level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Urea analyzes were performed in the laboratory.

  8. Change from the baseline serum creatinine level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Creatinine analyzes were performed in the laboratory.

  9. Change from the baseline serum total cholesterol level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Total cholesterol analyzes were performed in the laboratory.

  10. Change from the baseline serum low density lipoprotein level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Low density lipoprotein analyzes were performed in the laboratory.

  11. Change from the baseline serum high density lipoprotein level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. High density lipoprotein analyzes were performed in the laboratory.

  12. Change from the baseline serum triglyceride level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Triglyceride analyzes were performed in the laboratory.

  13. Change from the baseline serum total antioxidant level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Total antioxidant analyzes were performed in the laboratory.

  14. Change from the baseline serum total oxidant level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Total oxidant analyzes were performed in the laboratory.

  15. Change from the baseline heparin plasma thiobarbituric acid reactive substances level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

    Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

    Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Thiobarbituric acid reactive substances analyzes were performed in the laboratory.

Sponsors and collaborators

Lead sponsor

Eastern Mediterranean University

Other

Collaborators

  • Ege University

Registry information

Official study title

The Effect of an Acute Maximal Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism

Important dates

Study start
2019
Primary completion
2020
Study completion
2020
First posted
Nov 7, 2019
Registry last updated
Sep 9, 2020

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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