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Completed

NCT Number: NCT01728675

Eccentric Exercise and Oxidative Stress

The purpose of the present investigation is to compare the responses and adaptations of young and elderly individuals to repeated eccentric exercise in regards to muscle function and redox homeostasis in a side-by-side comparison.

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Key information

Conditions

Aging Muscle Damage Oxidative Stress

Age range

18 year–70 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

European University Cyprus

Nicosia, 1516, Cyprus

About this study

Despite the progress of analytic techniques and the refinement of study designs, striking disagreement exists among studies regarding the influence of exercise on muscle function and redox homeostasis in the elderly. A specific exercise protocol will be applied (the repeated eccentric model) to produce long-lasting and extensive changes in redox biomarkers and to examine more easily the potential effects of aging. Ten young men and ten elderly men will underwent an isokinetic eccentric exercise session, which will be repeated after three weeks. Muscle function indices and redox biomarkers will be assessed in urine, plasma or erythrocytes pre exercise, immediately post exercise, 2 and 4 days post exercise.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • subject provides written informed consent
  • physiological health profile
  • stable at their anthropometric characteristics for at least the last year

Exclusion criteria

  • muscle disease
  • prior history of musculoskeletal injury to the lower limbs
  • smokers
  • consumed any nutritional supplement the last 3 months
  • performed intense eccentric exercise the last 6 months
  • non Caucasian

Treatment and study plan

Exercise

Other

Participants will perform two isokinetic eccentric exercise bouts

Primary outcomes

  1. Muscle torque (torque)

    Time frame: Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

  2. Range of motion, ROM (degrees)

    Time frame: Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

  3. Delayed onset muscle soreness, DOMS (scale 1-10)

    Time frame: Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

  4. Creatine kinase, CK (activity IU)

    Time frame: Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

Secondary outcomes

  1. F2-isoprostanes (pg/mg creatinine)

    Time frame: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

  2. Protein carbonyls (nmol/mg protein)

    Time frame: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

  3. Glutathione, GSH (μmol/g Hb)

    Time frame: Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

  4. Catalase (U/g Hb)

    Time frame: Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

  5. Superoxide dismutase, SOD (U/g Hb)

    Time frame: Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

  6. Glutathione peroxidase, GPx (U/g Hb)

    Time frame: Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

  7. Glucose-6-phosphate dehydrogenase, G6PD (U/g Hb)

    Time frame: Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

  8. Uric acid (mM)

    Time frame: Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

  9. Albumin (μM)

    Time frame: Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

  10. Bilirubin (μM)

    Time frame: Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

Sponsors and collaborators

Lead sponsor

European University Cyprus

Other

Collaborators

  • Aristotle University Of Thessaloniki

Registry information

Official study title

The Effect of Eccentric Exercise on Oxidative Stress in the Elderly

Important dates

Study start
2012
Primary completion
2012
Study completion
2012
First posted
Nov 20, 2012
Registry last updated
Nov 20, 2012

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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