Skip to main content
OpenTrials
Completed

NCT Number: NCT06905964

Eccentric Cycling Exercise With Assistive Devices on Mitochondrial Metabolism in T Lymphocyte

Eccentric cycling exercise can be a promising modality fwith lower respiratory demands and higher power output. Assistive devices like neuromuscular electrical stimulation (NMES) and compressive garment (CG) accelerates muscle pumping and relieve the eccentric exercise-induced muscle soreness.

Phenotypes and mitochondrial metabolism are crucial to regulate acquired immnunity of T lymphocytes. The study aimed to investigate the effects of eccentric cycling exercise training combined with NMES and CG on mitochondrial phenotypes and bioenergetics in T lymphocytes in sedentary males. The hypothesis were an acute/intense exercise decreased the mitochondrial respiratory capacities of OXPHOS, ETS and bioenergetics, while eccentric cycling training could elevate the diminished mitochondrial functions.

Methods: Twelve sedentary healthy males were randomly assigned to either eccentric cycling with assistive devices (ECA, n=10) or eccentric cycling only (ECC, n=10). All participants performed ECA or ECC at an intensity of 60% to 120% maximal workload progressively on a costumed bicycle ergometer for 40 min/day, 5 days/week for 5 weeks. ECA performed NMES for 20min/session after cycling, while ECC was seated for resting 20 min. Systemic aerobic fitness, phenotypes of T lymphocytes, mitochondrial OXPHOS, ETS respiratory capacities, and bioenergetics in T lymphocytes were analyzed.

Completed

Looking for future studies?

Notify Me

Key information

Conditions

There Are no Conditions Under Study. Healthy Volunteers

Age range

20 year–30 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Chang Gung University

Guishan, Taoyuan, 333, Taiwan

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Sedentary lifestyle (exercise<20min/session; <1session/week for a year or longer).
  • Nonsmokers, nonusers of medications/vitamins.
  • No any cardiopulmonary, neuromuscular, and hematological risks.

Exclusion criteria

  • Have regular exercise habits (i.e., exercise frequency once per week, duration >20 min).
  • Have retabolic or musculoskeletal disease.

Treatment and study plan

Eccentric cycling exercise (ECC)

Behavioral

Performed exercise training 5 days a week for 5 weeks on an eccentric ergometer.

Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase.

After each training session, rest in sitting position for 20 min. Exercise intensity: Firstly, set at 80% Wmax, and progressively increased 5% per week to 120 % Wmax in the last week.

Eccentric cycling exercise with assistive devices (ECA)

Behavioral

Performed exercise training 5 days a week for 5 weeks on an eccentric ergometer.

Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase.

After each training session, receive NMES in sitting position for 20 min.

Intensity: Firstly, set at 80% Wmax, and progressively increased 5% per week to 120 % Wmax in the last week.

Primary outcomes

  1. Mitochondrial bioenergetics of oxygen consumption rate in T lymphocyte

    Time frame: At baseline and 2 days after completing the 5-week intervention

    The bioenergetic health index (BHI) of mitochindria in T lymphocyte was measured. BHI is a parameter for detecting the health status of mitochondrial and efficiency of energy metabolism.

  2. Systemic aerobic fitness (VO2)

    Time frame: At baseline and 2 days after completing the 5-week intervention

    VO2max was determined during the exercise testing. VO2max is the maximum of oxygen deliver and utilization.

Sponsors and collaborators

Lead sponsor

Chang Gung Memorial Hospital

Other

Registry information

Official study title

Effects of Eccentric Cycling Exercise Training With Assistive Devices on Phenotypes and Mitochondrial Metabolism in T Lymphocytes in Sedentary Men

Important dates

Study start
2023
Primary completion
2024
Study completion
2024
First posted
Apr 2, 2025
Registry last updated
Apr 2, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Related clinical trials

Published trials that share one or more normalized conditions with this study.