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Completed

NCT Number: NCT06576804

Eccentric Cycling Exercise on Mitochondrial Function of Lymphocyte

Eccentric cycling exercise (ECE) features lower metabolic demand and higher improvement of muscle strength compared to traditional concentric cycling exercise (CCE). Mitochondria can regulate energy metabolism and adaptive immune quality in T lymphocytes. However, the effects of ECE on mitochondrial functions in T-lymphocytes have not yet been established.

Method: A total of 33 healthy sedentary males were randomized and divided into ECE (n=11), CCE (n=11), and control groups (n=11). These subjects progressively performed CCE or ECE from 60% to 80% maximal workload on a bicycle ergometer for 40 min/day, 5 days/week for 6 weeks. A graded exercise testing and an isokinetic strength test were conducted to evaluate cardiopulmonary fitness and muscle strength, respectively. Phenotypes and mitochondrial respiratory capacity in T lymphocyte were analyzed using flow cytometry and high-resolution respirometer, respectively.

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Key information

Conditions

Eccentric Exercise Training

Age range

20 year–30 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Chang Gung University

Taoyuan, 333, Taiwan

About this study

Eccentric exercise training (EET) increases physical performance while having lower metabolic demand than concentric exercise training (CET).

Mitochondria of lymphocytes are essential for cell proliferation, death, and differentiation, and play a critical role in establishing lymphocyte phenotypes and their functions.

Whether EET influences lymphocyte bioenergetic efficiency remains unclear. The study was to investigate the effects of ECE and CCE regimens on adaptive immune functions and mitochondrial bioenergetics of T lymphocytes in sedentary males.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Sedentary lifestyle.
  • Nonsmokers, nonusers of medications/vitamins.
  • No any cardiopulmonary/hematological risks.

Exclusion criteria

  • Have regular exercise habits (i.e., exercise frequency once per week, duration >20 min).
  • Have retabolic or musculoskeletal disease.

Treatment and study plan

Eccentric cycling training

Behavioral

Performed exercise training 5 days a week for 6 weeks on an eccentric ergometer.

Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase.

Intensity: Firstly, set at 60% Wmax, and progressively increased 5% per week

Concentric cycling training

Behavioral

Performed exercise training 5 days a week for 6 weeks on an eccentric ergometer.

Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase.

Intensity: Firstly, set at 60% Wmax, and progressively increased 5% per week

Primary outcomes

  1. Mitochondrial function of lymphocyte

    Time frame: 12 weeks

    Evaluate bioenergetic health index (BHI) of mitochondria in lymphocyte by using a high resolution respirometer.

Secondary outcomes

  1. Cardiopulmonary fitness

    Time frame: 12 weeks

    The graded exercise test (GXT) on a bicycle ergometer was performed. The GXT comprised 2 min of unloaded pedaling followed by a continuous increase in the work rate of 30 watt per 3-minute until exhaustion (i.e., progressive exercise to maximal O2 consumption)

Sponsors and collaborators

Lead sponsor

Chang Gung Memorial Hospital

Other

Registry information

Official study title

Effects of Concentric and Eccentric Exercise Regimens on Bioenergetic Efficiency of Lymphocytes in Sedentary Males

Important dates

Study start
2021
Primary completion
2022
Study completion
2022
First posted
Aug 29, 2024
Registry last updated
Aug 29, 2024

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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