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NCT Number: NCT07145814

Dexmedetomidine Dose-Dependent on Lung Function and AQP1 in One-Lung Ventilation Surgery

This randomized controlled trial evaluated the dose-dependent effects of dexmedetomidine (DEX) on pulmonary function and aquaporin-1 (AQP1) expression during one-lung ventilation (OLV) in thoracoscopic surgery.

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Key information

Conditions

Acute Lung Injury(ALI) Acute Lung Injury Lung Diseases Lung Injury Respiratory Tract Diseases

Age range

40 year–70 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Department of Anesthesiology, Nanxishan Hospital of Guangxi Zhuang Autonomous Region

Guilin, Guangxi, 541002, China

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • American Society of Anesthesiologists (ASA) physical status I or II
  • Age 40-70 years
  • Body mass index (BMI) 18-30 kg/m²

Exclusion criteria

  • Preoperative tumor radiotherapy or chemotherapy
  • Immune or endocrine disorders
  • Hepatic or renal dysfunction
  • Preoperative corticosteroid use
  • Recent respiratory infection or chronic lung disease
  • Sick sinus syndrome or atrioventricular block
  • Preoperative anemia (hemoglobin < 90 g/L)

Treatment and study plan

dexmedetomidine 0.3 µg/kg/h

Drug

Following Anesthesia induction, dexmedetomidine (DEX) Low Dose group received a DEX loading dose (0.5 µg/kg over 10 min), followed by continuous infusions of 0.3 µg/kg/h, respectively, until 30 minutes before the end of the operation.

dexmedetomidine 0.5 µg/kg/h

Drug

Following Anesthesia induction, dexmedetomidine (DEX) high dose group received a DEX loading dose (0.5 µg/kg over 10 min), followed by continuous infusions of 0.5 µg/kg/h, respectively, until 30 minutes before the end of the operation.

0.9 % saline

Drug

Placebo group received an equivalent volume of 0.9% saline.

Primary outcomes

  1. aquaporin-1 (AQP1) expression during one-lung ventilation in thoracoscopic surgery

    Time frame: before one-lung ventilation, Immediately after lobectomy

    AQP1 expression, serving as an indicator of pulmonary interstitial water permeability, was assessed by immunohistochemistry (IHC) on lung tissue samples. Two histologically normal specimens (1×1×1 cm³ each) were collected per patient: 1) Before one-lung ventilation: ≥ 5 cm from tumor margin in planned resection area; 2) Immediately after lobectomy: ≥ 5 cm from margin in isolated lung. Tissues were fixed in 10% neutral buffered formalin (24 hours), paraffin-embedded, and sectioned (3 μm). Five random high-power fields (HPF, 400x) per section were evaluated. The percentage of positive cells (0: 0-25%; 1: 26-50%; 2: 51-75%; 3: 76-100%) and staining intensity (0: none; 1: light yellow; 2: brown; 3: dark brown) were scored. The total IHC score (range 0-9) was calculated as (percentage score x intensity score); specimens were classified as low (≤ 4) or high (> 4) AQP1 expression.

Secondary outcomes

  1. Pulmonary function during one-lung ventilation in thoracoscopic surgery, including lung compliance (Cdyn)

    Time frame: pre-dexmedetomidine administration, 60 minutes, 90 minutes, 120 minutes after one-lung ventilation initiation, and 30 minutes after reinstitution of two-lung ventilation

    Radial arterial blood samples were drawn for blood gas analysis at the specified time points. Dynamic lung compliance (Cdyn) was recorded from the anesthesia machine.

  2. Pulmonary function during one-lung ventilation in thoracoscopic surgery, including respiratory index (RI), and oxygenation index (OI).

    Time frame: pre-dexmedetomidine administration, 60 minutes, 90 minutes, 120 minutes after one-lung ventilation initiation, and 30 minutes after reinstitution of two-lung ventilation

    Radial arterial blood samples were drawn for blood gas analysis at the specified time points. The respiratory index (RI) and oxygenation index (OI) were calculated as:

    RI=P(A-aDO2)/PaO2=[(Pb-PH2O) × FiO2-PaO2-PaCO2/RQ]/PaO2. OI= PaO2/FiO2. P(A-aDO2) - alveolar-arterial blood oxygen partial pressure difference, PaCO2 - partial arterial pressure of CO2, Pb - barometric pressure (760 mmHg), pH2O - partial pressure of water vapor (47 mmHg), RQ - respiratory quotient (0.8).

Sponsors and collaborators

Lead sponsor

People's Hospital of Guangxi Zhuang Autonomous Region

Other

Registry information

Official study title

Dexmedetomidine Dose-Dependent Modulation of Lung Function and AQP1 Expression During One-Lung Ventilation in Adults Undergoing Thoracoscopic Surgery: A Randomized Controlled Trial

Important dates

Study start
2018
Primary completion
2019
Study completion
2019
First posted
Aug 28, 2025
Registry last updated
Aug 28, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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