Development of 4D Flow MRI for Risk Stratification of Variceal Bleeding in Cirrhosis

The overall goal of this research is to implement advanced non-invasive 4D flow MRI biomarkers to predict the presence of treatable but potentially lethal GEV in patients with cirrhosis. This would facilitate the triage of patients with high-risk GEV to therapeutic EGD, while reducing unnecessary EGD procedures in patients without them.

The primary biological mechanism for development of GEV is elevated portal pressure and reversal of flow in the left gastric vein (LGV). Applying 4D flow MRI, investigators aim to detect and quantify reversed flow in the LGV to detect GEV at risk for bleeding.

Aim 1: Perform pre-clinical validations of an optimized, accelerated radial 4D flow MRI strategy, and of fat mitigation strategies for radial 4D flow MRI.

Aim 2: Determine the diagnostic performance of radial 4D flow MRI, in cirrhotic adults including

• diagnostic accuracy to identify high-risk GEV using EGD as reference standard, and
• test-retest repeatability Aim 3: Evaluate the effects and added value of a meal challenge to assess for high-risk GEV.

Aim 4: Compare the accuracy of 4D flow MRI to current non-invasive markers of liver disease.

Aim 5: Determine the feasibility of radial 4D flow MRI in participants with FALD to detect differences in mesenteric response to a meal challenge when compared to healthy controls and participants with other types of liver disease.

Research Procedures

Pre-Clinical Validation (Phase 1): A total of 21 participants (7 healthy volunteers, 14 patients with GEV) to evaluate the optimized 4D flow methods, and 20 obese subjects to evaluate fat-mitigation strategies, will be enrolled. Participants will be asked to complete a single research visit that will include a contrast enhanced MRI scan lasting up to 1 hour. Participants will be asked to fast for at least 5 hours prior to the exam. Participants will be screening a final time for contraindications to contrast enhanced MR imaging; an IV will be placed; and participants will be positioned in the MR scanner, asked to lie as still as possible and to follow some breath hold instructions.

Clinical Validation (Phase 2-3): A total of 105 patients diagnosed with cirrhosis will be enrolled. Participants will be asked to complete a single research visit, lasting approximately 2 hours, that will include the following procedures:

• Participants will be asked to fast for 12 hours prior to arriving.
• An IV will be placed for tracer administration
• Participants will undergo a research MRI lasting approximately 1.5 hours (up to 1 hour of total scan time)

• All participants will be positioned in the MRI scanner for the initial scanning session (30 min) during which the first dose of GBCA (3/4 of total dose) or the total dose of Ferumoxytol will be administered.
• 50 participants will be removed from the scanner bore, repositioned, and scanned for an additional 15 minutes (repeatability testing).
• All participants will then be removed from the scanner and asked to consume 16 ounces of Ensure Plus®. After 20 minutes, they will be repositioned in the scanner for an additional scanning session (15 min) during which, a second dose of GBCA (1/4 of total dose) will be administered if required.

Inclusion criteria

for Aim 1:

• Healthy volunteers: Adults (>18 years) with no known liver pathology
• Obese volunteers: Adults (>18 years), no known liver pathology, body mass index (BMI) ≥ 35
• Patients: Adults (>18 years) with known cirrhosis and known Gastroesophageal varices

Exclusion criteria

for Aim 1:

• contraindications to MRI
• hypersensitivity reactions to both contrast agents
• patients with recent treatment for varices with embolization, TIPS, or other endovascular treatment
• patients with active GEV bleeding; known occlusive thrombus in portal vein, splenic vein, or superior mesenteric vein.
• patients with large HCC with known PC involvement.

Inclusion criteria

for Aim 2-4:

• Adults (>18 years) with known cirrhosis

Exclusion criteria

for Aim 2-4:

• Contraindications to MRI
• Recent treatment (< 1 year) for varices
• Known occlusive thrombus in portal vein; splenic vein, or superior mesenteric vein
• Large hepatocellular carcinoma (HCC) with known PV involvement
• Hypersensitivity reactions to both contrast agents
• Participants requiring conscious sedation for imaging are not eligible; participants requiring mild, oral anxiolytics for the clinical MRI scan will be allowed to participate as long as the following criteria are met:

• The participant has their own prescription for the medication
• The informed consent process is conducted prior to the self-administration of the medication.
• The participant comes to the research visit with a driver.

Inclusion criteria

for Aim 5:

• Adults (>18 years) with Fontan repair and diagnosed FALD

Exclusion criteria

for Aim 5:

• Contraindications to MRI
• Recent treatment (< 1 year) for varices
• Known occlusive thrombus in portal vein; splenic vein, or superior mesenteric vein
• Large hepatocellular carcinoma (HCC) with known PV involvement
• Hypersensitivity reactions to both contrast agents
• Participants requiring conscious sedation for imaging are not eligible; participants requiring mild, oral anxiolytics for the clinical MRI scan will be allowed to participate as long as the following criteria are met:

• The participant has their own prescription for the medication
• The informed consent process is conducted prior to the self-administration of the medication.
• The participant comes to the research visit with a driver.