Detection of Cav1 Calcium Channels in Inflammatory Cells of Asthmatic Children

Inclusion criteria

For Asthmatic children arm :

• Girl not in childbearing potential or taking contraceptive treatment
• Asthma diagnosis in at least 6 months as defined by clinical symptoms (expiratory dyspnea, wheezing, sibilance) and respiratory function (reversibility of VEMS at least 12% after salbutamol administration)
• Allergologic tests positives to airborne allergens : positive prick test and/or specific IgE > 0,35 kU/L for at least one allergen
• Asthma severity between mild to moderate based on Global initiative for asthma classification during more than one month with inhaled corticotherapies +/- long term action beta -mimetic long compounds (salmeterol, formoterol)
• Patient insured with a social security scheme

For control children arm :

• Girl not in childbearing potential or taking contraceptive treatment
• No medical history of asthma
• No medical history of allergy or symptoms of allergy on skin (atopic dermatitis), respiratory tract (rhino-conjunctivitis, asthma) or food allergy

Exclusion criteria

For both arms:

• Chronic respiratory pathology : known occlusive or restrictive respiratory syndrome like interstitial lung disease, neuromuscular disease, mucoviscidoses, respiratory tract abnormalities with cough, dyspnea or digital hippocratism.
• History of pulmonary or nose and throat infectious disease during last 4 months
• Parasitic disease non treated during last 3 months
• Dyspnea during the inclusion
• Systemic corticotherapy during the last 3 months before inclusion
• Immunosuppressive therapy during the last 3 months before inclusion
• Diabeta
• Disease causing immune disorder
• Infection during inclusion
• Participation to another study witch implies medical treatment during last 3 months before inclusion

For asthmatic children arm :

• Chronic pulmonary pathology (excluding asthma)
• Asthma exacerbation during inclusion
• VEMS < 70% of normal range or VEMS < 70% of normal range after inhalation of serum (despite salbutamol administration)
• Patient unable to complete pulmonary function testing