Dental Plaque Microbiota in Caries-free and Caries-active Children

The objective of this study will be to investigate the dental plaque's microbial diversity using Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) technology in the mixed dentition as well as the levels of salivary proteins in caries-free compared with caries active Arab children. The study protocol was approved by the Health Science Center Ethical Committee at Kuwait University.

One hundred 6-9 year-old children with caries active and caries-free status will be recruited from randomly selected public schools in Kuwait. After DNA purification from supragingival plaque samples, HOMINGS will be performed. To get an insight into the host factors involved in this complex interplay between the host and the bacteria, stimulated saliva from the study subjects will be analyzed by proteomics. For children in both groups supragingival plaque samples will be collected from the buccal and lingual surfaces of all teeth. After preparing the samples, the aliquots of purified DNA samples will be shipped to The Forsyth Institute for Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) protocol.

Inclusion criteria

• Arab 6-9 year old children
• Healthy
• Caries free, dmft/DMFT=0
• Caries Active: dmft/DMFT>5

Exclusion criteria

• Children with a systemic disease or any drug use that could affect the microflora of the oral cavity or the salivary gland functions
• History of salivary gland diseases
• Those who received antibiotic therapy during the last 3 months
• Children with severe localized or generalized periodontitis.