Cutaneous DNA Damage Caused by UV-A Irradiation

Due to their capacity to damage Deoxyribonucleic acid (DNA), Ultra-Violet (UV) radiation is one of the causes of skin cancers.

Until recently, the genotoxic effects of UV-A radiation, were poorly identified, in particular their capacity to lead to the dimerization of pyrimidine bases .

It is well known that the response to UV-A and UV-B radiations is different depending on the cutaneous phototype.

Thus, the aim of this study is to determine the correlation between cutaneous phototype and the quantity and nature (CPD or oxidative lesions) of damage caused to cutaneous DNA after an ex-vivo exposure to UV-A and UV-B radiations.

Inclusion criteria

• Male,
• Between 18 and 35 years old,
• Healthy volunteers,
• Cutaneous phototype 2 or 4 according to the Fitzpatrick classification,
• Affiliation to the French Social Security.

Exclusion criteria

• History of photosensibility,
• Active smoking or stopped since less than one year,
• Dermatological pathology or treatment contra-indicating cutaneous irradiation and skin biopsies,
• Any chronic pathology susceptible to interfere with the evaluations related to the protocol,
• Allergy to local anaesthetics,
• Volunteers who take drugs and/or food complements acting on oxidative stress in the 8 weeks preceding inclusion,
• Volunteers who have take paracetamol or aspirin within 7 days prior to the inclusion visit,
• Subject in exclusion period for another biomedical research study,
• Subject having exceeded the threshold of annual compensation for biomedical research.