Cushing's Syndrome Before and After Treatment (CORRECT)

Questionnaire filled out by patients

Blood pressure, both standard office readings and as AOBP (Automated Office Blood Pressure)

Arterial stiffness is assessed as Carotid-femoral PWV (cfPWV), measured using the SphygmoCor device (version 9; AtCor Medical

Endothelial function will be assessed by the EndoPAT® device (Itamar Medical)

Assessed through fasting blood levels of insulin and glucose (Homeostasis Model Assessment, HOMA)

Assessed as glycated hemoglobin A1C levels measured at three time points

Messenger RNA expression of core clock genes such as BMAL1, CLOCK, PER1-3, CRY1-2, REV-ERBα -β and -γ, RORA-C will be determined. For comparison, housekeeping genes such as Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β2 microglobulin (B2M) and β-actin (ACTB) will be used.

All will be expressed as -fold change from baseline expression levels

Dual X-ray absorptiometry (DXA), is used to measure whole body lean body mass, and fat mass in gram

Dual X-ray absorptiometry (DXA), bone mineral density (BMD) of the lumbar spine and femoral neck.

Dual X-ray absorptiometry (DXA), is used to assess vertebral fractures (VFA).

Change in weight in kilograms

Height and weight are combined to express body mass index (BMI, kg/m2)

waist circumference is recorded with measuring tape.

Hip circumference is recorded with measuring tape.

The TUG Test combines measures of physical performance and coordination. The TUG test measures the time to stand up, walk 3 m in a straight line, and immediately return to the chair.

The result is expressed as the fastest time in seconds

The short physical performance battery (SPPB) is a group of bed-side measures that combines the results of walking speed, chair stand and balance tests.

The result is expressed as a collective score from 0-12

Handgrip strength is measured using a hand dynamometer with the participant seated

Measured with an Actigraph tri-axial accelerometer on the non-dominant wrist. Expressed as Counts/day

Measured with an Actigraph tri-axial accelerometer on the non-dominant wrist. Expressed as hours/day

Measured with an Actigraph tri-axial accelerometer on the non-dominant wrist. Expressed as hours/day

The questionnaire records types and intensity of physical activity and consists of seven open items

Flow assisted cell sorting (FACS) will be performed on blood leukocytes followed by real time quantitative polymerase chain reaction on distinct cells or in single cell suspension from relevant tissue.

free cortisol

Steroid metabolome

Adrenergic metabolites

Questionnaire filled out by patients

Questionnaire filled out by patients

Such as ADAM17 (Tumor necrosis factor-α-converting enzyme - TACE) Soluble CD16 (Human Low affinity immunoglobulin gamma Fc region receptor III-A) Fibrinogen vWF D-dimer Plasminogen, plasminogen activator inhibitor 1 Plasmin inhibitor

Such as plasma levels of Adiponectin, Leptin, Free fatty acids, cholesterol

Such as Interleukin 6 Interleukin 10 Tumor necrosis factor alfa Transforming growth factor alfa

Measured with cytokine luminex

Assessed via electronic patient records