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NCT Number: NCT05185167

Comparison of the Genotoxicity of Desflurane and Propofol

The purpose of this study is to evaluate if the anesthetics propofol and desflurane can damage DNA according to comet essay in patients submitted to lumbar disc surgery.

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Key information

Conditions

DNA Damage Anesthesia

Age range

18 year–65 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Mugla Sitki Kocman University

Muğla, 48000, Turkey (Türkiye)

About this study

It is expected that DNA damage information provide the possible genotoxic effects of inhalational and intravenous anesthetics and contribute to the establishment of strategies aimed at reducing the risks to exposed patients.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Ability to understand and willingness to sign an informed consent form
  • Elective lumbar spinal surgery that will take at least 120 minutes,
  • American Society of Anesthesiologists (ASA) physical status I and II

Exclusion criteria

  • Patients with ASA physical status III or IV disease
  • Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders)
  • Smoking or alcohol consumption
  • Recently received radiation, chemotherapy
  • Malignancy
  • Patients who will receive blood transfusion during the operation
  • Users of antioxidant supplements
  • Patients who have received general anesthesia in the last three months
  • Patients with known occupational exposures (operating room personnel, chemical plant worker

Treatment and study plan

Anesthetics, General Desflurane Maintenance

Drug

Comparison of dna damage of desflurane maintenance with propofol maintenance according to comet analysis

Anesthetics, General Propofol Maintenance

Drug

Comparison of dna damage of propofol maintenance with desflurane maintenance according to comet analysis

Primary outcomes

  1. Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

    Time frame: Before anesthesia induction

    The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.

  2. Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

    Time frame: 2 hours after anesthesia induction

    The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.

  3. Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

    Time frame: postoperative 1st day

    The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.

  4. Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

    Time frame: postoperative 5th day

    The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.

  5. Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

    Time frame: Before anesthesia induction

    The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.

  6. Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

    Time frame: 2 hours after anesthesia induction

    The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.

  7. Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

    Time frame: postoperative 1 st day

    The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.

  8. Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

    Time frame: postoperative 5th day

    The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.

  9. Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

    Time frame: before anesthesia induction

    The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.

  10. Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

    Time frame: 2 hours after anesthesia induction

    The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.

  11. Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

    Time frame: postoperative 1 st day

    The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.

  12. Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

    Time frame: postoperative 5th day

    The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.

Sponsors and collaborators

Lead sponsor

Muğla Sıtkı Koçman University

Other

Registry information

Official study title

Genotoxic Effects of Desflurane and Propofol Anesthesia in Patients Undergoing Lumbar Disc Surgery

Important dates

Study start
2022
Primary completion
2022
Study completion
2022
First posted
Jan 11, 2022
Registry last updated
Mar 31, 2022

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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