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NCT Number: NCT07531992

Comparison of Heart Rate Variability Analysis Durations and Their Relationship With Blood Pressure

This observational study aims to compare heart rate variability (HRV) parameters obtained from different analysis durations in healthy adults and to examine how these parameters relate to resting blood pressure values. HRV is a noninvasive measure commonly used to assess autonomic nervous system activity. Standard short-term HRV analysis is typically based on 5-minute recordings, but shorter and longer analysis durations are also used in research and practice. This study will evaluate whether HRV values derived from 1-minute, 5-minute, 10-minute, and 20-minute analysis windows differ from one another and whether their relationships with systolic blood pressure, diastolic blood pressure, and mean arterial pressure change according to analysis duration. Participants will attend a single study visit. After a rest period, resting blood pressure and pulse will be measured, and a continuous HRV recording will be obtained using a chest strap device. The study is designed to provide methodological information on the comparability of different HRV analysis durations under the same physiological conditions.

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Key information

Conditions

Heart Rate Variability

Age range

18 year–40 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Artvin Coruh University, Artvin Vocational School, Disabled Care and Rehabilitation Laboratory, Artvin,

Artvin, Turkey (Türkiye)

Location contact

Ömer Dicle Kızıl, MSc

CONTACT

[email protected]

+90 541 523 36 91

Ömer Dicle Kızıl, MSc

SUB_INVESTIGATOR

About this study

Heart rate variability (HRV) is widely used as a noninvasive indicator of autonomic nervous system regulation and provides information about sympathetic-parasympathetic balance. Although 5-minute recordings are commonly recommended for short-term HRV assessment, interest has increased in ultra-short and longer analysis durations because they may improve feasibility and reduce participant burden. However, the extent to which HRV parameters derived from different analysis durations agree with one another remains uncertain, particularly when comparing time-domain and frequency-domain measures. In addition, limited research has systematically examined whether the relationship between HRV and blood pressure varies according to HRV analysis duration. This study is intended to address that methodological gap.

This is a single-center, single-visit, observational methodological comparison study in healthy adults. Eligible participants will complete a sociodemographic data form and will undergo resting hemodynamic assessment and HRV recording under standardized conditions. Measurements will be performed in a quiet environment after an adaptation period. Resting systolic blood pressure, diastolic blood pressure, and pulse will be measured using an automated oscillometric upper-arm blood pressure monitor. RR interval data will then be recorded continuously using the Polar H10 chest strap and analyzed using Kubios HRV software.

HRV analyses will be derived from a single continuous recording using predefined analysis windows of 1 minute, 5 minutes, 10 minutes, and 20 minutes. These windows will be extracted from the same physiological recording in order to minimize time-related variation and allow direct comparison across durations. Time-domain and frequency-domain HRV parameters will be calculated, and the agreement and comparability of measurements across analysis durations will be evaluated. Associations between HRV parameters and resting hemodynamic variables, including systolic blood pressure, diastolic blood pressure, mean arterial pressure, and pulse, will also be examined according to analysis duration.

The study is expected to provide methodological evidence regarding whether shorter or longer HRV analysis durations can be interpreted consistently relative to standard short-term recordings. The findings may help improve protocol standardization and support more accurate physiological and clinical interpretation of HRV data obtained under resting conditions.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Healthy adults aged 18 to 40 years
  • Willing and able to provide informed consent
  • Able to comply with pre-assessment instructions, including restrictions related to caffeine intake and strenuous physical activity
  • No condition preventing heart rate variability and blood pressure measurements

Exclusion criteria

  • History of diagnosed cardiovascular, neurological, or autonomic nervous system disease
  • Known arrhythmia or pacemaker use
  • Regular use of medications that may affect heart rate variability, such as beta blockers or antiarrhythmic drugs
  • Acute infection, fever, or any condition affecting general health status on the day of assessment
  • Severe motion artifact during measurement or technically unusable recordings

Treatment and study plan

Heart Rate Variability and Blood Pressure Assessment

Other

Participants will complete a single-visit resting physiological assessment under standardized conditions. After an adaptation period, systolic blood pressure, diastolic blood pressure, and pulse will be measured using an automated oscillometric upper-arm blood pressure monitor. Continuous RR interval data will then be recorded using the Polar H10 chest strap and analyzed with Kubios HRV software. Heart rate variability parameters will be calculated from predefined 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous recording for methodological comparison.

Primary outcomes

  1. RMSSD Across Different Analysis Durations

    Time frame: Baseline, during a single study visit

    Root mean square of successive differences (RMSSD), in milliseconds (ms), calculated from predefined 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording obtained under standardized resting conditions.

  2. SDNN Across Different Analysis Durations

    Time frame: Baseline, during a single study visit

    Standard deviation of normal-to-normal intervals (SDNN), in milliseconds (ms), calculated from predefined 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording obtained under standardized resting conditions.

  3. Low-Frequency Power Across Different Analysis Durations

    Time frame: Baseline, during a single study visit

    Low-frequency (LF) power, in ms^2, calculated from predefined 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording obtained under standardized resting conditions.

  4. High-Frequency Power Across Different Analysis Durations

    Time frame: Baseline, during a single study visit.

    High-frequency (HF) power, in ms^2, calculated from predefined 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording obtained under standardized resting conditions.

  5. LF/HF Ratio Across Different Analysis Durations

    Time frame: Baseline, during a single study visit.

    Low-frequency/high-frequency (LF/HF) ratio calculated from predefined 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording obtained under standardized resting conditions.

Secondary outcomes

  1. Agreement of RMSSD Across Different Analysis Durations

    Time frame: Baseline, during a single study visit

    Agreement of RMSSD values, in milliseconds (ms), obtained from 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording, assessed using intraclass correlation coefficients and Bland-Altman analysis.

  2. Agreement of SDNN Across Different Analysis Durations

    Time frame: Baseline, during a single study visit

    Agreement of SDNN values, in milliseconds (ms), obtained from 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording, assessed using intraclass correlation coefficients and Bland-Altman analysis.

  3. Agreement of Low-Frequency Power Across Different Analysis Durations

    Time frame: Baseline, during a single study visit

    Agreement of low-frequency (LF) power values, in ms^2, obtained from 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording, assessed using intraclass correlation coefficients and Bland-Altman analysis.

  4. Agreement of High-Frequency Power Across Different Analysis Durations

    Time frame: Baseline, during a single study visit

    Agreement of high-frequency (HF) power values, in ms^2, obtained from 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording, assessed using intraclass correlation coefficients and Bland-Altman analysis.

  5. Agreement of LF/HF Ratio Across Different Analysis Durations

    Time frame: Baseline, during a single study visit

    Agreement of low-frequency/high-frequency (LF/HF) ratio values obtained from 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording, assessed using intraclass correlation coefficients and Bland-Altman analysis.

  6. Correlation Between RMSSD and Resting Systolic Blood Pressure

    Time frame: Baseline, during a single study visit

    Correlation between RMSSD, in milliseconds (ms), and resting systolic blood pressure, in mmHg, measured under standardized resting conditions during the same study visit.

  7. Correlation Between RMSSD and Resting Diastolic Blood Pressure

    Time frame: Baseline, during a single study visit

    Correlation between RMSSD, in milliseconds (ms), and resting diastolic blood pressure, in mmHg, measured under standardized resting conditions during the same study visit.

  8. Correlation Between RMSSD and Mean Arterial Pressure

    Time frame: Baseline, during a single study visit

    Correlation between RMSSD, in milliseconds (ms), and mean arterial pressure, in mmHg, calculated from resting blood pressure measurements obtained during the same study visit.

  9. Resting Systolic Blood Pressure

    Time frame: Baseline, during a single study visit

    Resting systolic blood pressure, in mmHg, measured under standardized resting conditions before HRV recording.

  10. Resting Diastolic Blood Pressure

    Time frame: Baseline, during a single study visit

    Resting diastolic blood pressure, in mmHg, measured under standardized resting conditions before HRV recording.

  11. Mean Arterial Pressure

    Time frame: Baseline, during a single study visit

    Mean arterial pressure, in mmHg, calculated from resting systolic and diastolic blood pressure measurements obtained under standardized resting conditions before HRV recording.

  12. Resting Pulse Rate

    Time frame: Baseline, during a single study visit

    Resting pulse rate, in beats per minute, measured under standardized resting conditions before HRV recording.

Study contacts

Contact information is provided by the study sponsor or research team.

Sefa Haktan Hatık, PhD

CONTACT

[email protected]

+90 368 271 55 28

Sponsors and collaborators

Lead sponsor

Sinop University

Other

Registry information

Official study title

Comparison of Heart Rate Variability Analysis Durations and Their Relationship With Blood Pressure: A Methodological Comparative Study

Acronym: HRV-BP

Important dates

Study start
2026
Primary completion
2026
Study completion
2026
First posted
Apr 15, 2026
Registry last updated
Apr 15, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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