A proforma will be prepared by researcher and finalized after pre-testing. The patients admitted in Sahiwal Teaching Hospital, Sahiwal will be assessed by the researcher himself and results will be noted on the proforma. Patients will be admitted to hospital one day before surgery. Detailed history of urinary symptoms, sexual function, catheterization, instrumentation, urinary tract infection, trauma will be taken to obtain the cause of stricture. Demographic information like name, age, and contact number will be recorded. Investigations will include preoperative complete blood count, renal function test, liver function test, urine complete examination, ultrasonogram kidney, ureter and bladder with post void residual volume (PVR), uroflowmetry and retrograde urethrogram for diagnosis and length of stricture. A midline perineal incision will be made in lithotomy position from the perineoscrotal junction to about 1 cm from the anal margin. Incision will be deepened through the subcutaneous tissue and Colles' fascia to expose the bulbospongiosus muscle. The bulbospongiosus muscle will be divided in the midline and retracted. In Group A (Excision and Primary anastomosis urethroplasty) patients, an incision will be made down onto the catheter in the ventral midline. Urethra will be divided with scissors proximally and distally until healthy urethra is entered. Stay sutures will be placed. The scarred urethra will be excised, and the healthy proximal urethral segment will be spatulated dorsally so that it accommodates a 30 French bougie and the distal urethra will then be spatulated ventrally. The anastomosis will be done using interrupted sutures of 4-0 polydioxanone (PDS). While in Group B (Tunica Vaginalis Graft Urethroplasty) patients, an incision will be made onto the urethra in the ventral mid-line along the stricture length, opening into healthy proximal and distal urethra and stay sutures will be positioned. Tunica vaginalis graft is harvested by making a small vertical or transverse hemiscrotal incision. The dartos fascia is divided to expose the tunica vaginalis. The testis is gently delivered through the incision. Graft outlined on the parietal tunica vaginalis and carefully dissected avoiding injury to the testis or epididymis. Hemostasis is secured. The harvested tunica vaginalis graft is placed on a sterile surface with the serosal surface facing upward. It is then trimmed to the required size. The graft is kept moist in normal saline until applied to the urethral site. The tunica vaginalis defect is approximated and testis is placed back into the scrotum. The dartos and skin are closed in layers using absorbable sutures (Mridha et al., 2023). The graft will be laid as a ventral onlay graft onto the opened urethral defect and sutured to the urethral mucosal edges using absorbable sutures. Quilting or anchoring sutures may be placed to reduce graft dead space and enhance take (Siegel et al., 2024). Ventral urethrotomy is closed over the catheter using absorbable sutures. A 16 French soft silicone catheter will be kept in place for 2-3 weeks for drainage of urine. The bulbospongiosus muscle will be closed in the midline using interrupted 3-0 vicryl sutures. A suction drain is placed in the perineum overlying the muscle, and Colles' fascia is closed using running 3-0 vicryl, followed by skin closure with running 4-0 chromic. A clear adhesive dressing is placed, and the patient is placed supine. Per urethral foley catheter will be removed 2-3 weeks after the procedure. All procedures will be performed by experienced urologists using standardized surgical techniques to ensure consistency. Postoperative follow-ups will be conducted at scheduled intervals as per the prescribed proforma. Data will be collected through proforma.
