CD71 in Dried Blood Spots in Healthy Males

Despite being banned by the World Anti-Doping Agency, blood doping is a common method of performance enhancement used by athletes wishing to gain an unfair advantage over their competition. A common way to achieve this increase is by using erythropoiesis stimulating agents (ESA's), namely recombinant erythropoietin (rEPO). Though laboratory tests have been developed for the direct detection of all known isoforms of exogenously administered ESAs in both urine and blood, athletes have found ways to circumvent these testing measures using techniques such as microdosing.

Inclusion criteria

Active individuals, preferably those that participate regularly in endurance athletics either for sport or for leisure, between the ages of 18 and 45

• Participants should have ferritin > 35 ng/mL and transferrin saturation > 20% at the time of enrollment

Exclusion criteria

Individuals currently enrolled in a registered testing pool for anti-doping purposes

• Individuals with the intent to compete in sanctioned athletic events during the study period
• Unwillingness to provide urine samples or blood samples
• Not actively exercising
• Individuals who show a high risk for MI/CAD, stroke, CHF, and venous thromboembolism (VTE)., as defined by the Principal Investigator
• Individuals with known drug allergies
• Individuals with EKG abnormalities, as determined by the Principal Investigator
• Individuals who have chronic kidney disease, HIV, cancer, hepatitis B, hepatitis C, or are planning surgery during the study
• Individuals with history of acute or chronic medical or psychiatric condition
• GFR (Creatinine clearance) <60 mL/min
• Ferritin >270 ng/mL
• Individuals who have a baseline hemoglobin concentration greater than 15.5 g/dL or a baseline hematocrit above 47%
• Individuals with blood or iron disorders, including polycythemia, hemochromatosis, anemia, or iron-deficiency anemia
• Individuals with a history of bleeding or bone marrow aplasia
• Individuals who are diabetic or with a history of cardiac or hepatic disease or history of drug abuse