Cardioprotective Effects of Omega-3 Fatty Acids Supplementation in Healthy Older Subjects Exposed to Air Pollution Particles

Inclusion criteria

• Age 50-75 years old generally healthy male and female.
• Normal resting electrocardiogram.
• Oxygen saturation greater than 94% at the time of physical exam.

Exclusion criteria

• A history of angina, cardiac arrhythmias, congestive heart failure, and ischemic myocardial infarction or coronary bypass surgery.
• Cardiac pacemaker.
• Uncontrolled hypertension (> 150 systolic, > 90 diastolic).
• Neurodegenerative diseases such as Parkinson's and Alzheimer disease.
• A history of chronic illnesses such as diabetes, cancer (possible exception for history of nonmelanoma skin cancer), rheumatologic diseases, immunodeficiency state, significant chronic respiratory diseases such as chronic obstructive pulmonary disease or severe asthma.
• A history of migraine headache.
• History of bleeding diathesis.
• Currently taking beta-blockers to control hypertension and/or arrhythmias.
• Use of oral anticoagulants.
• Participants must refrain from all over-the-counter nonsteroidal anti-inflammatory drugs for a period of two weeks prior to exposure. Low-dose aspirin will be acceptable. Medications not specifically mentioned here may be reviewed by the investigators prior to a participant's inclusion in the study.
• Allergies to fish or omega-3 fatty acids.
• Subjects are on prescriptions taking omega-3 fish oil as therapy.
• Subjects will be required to avoid taking omega-3 fatty acids or having more than one 4-6 oz/serving of all types of fish and shellfish, walnuts, flaxseeds and flaxseed oil, rapeseed oil, canola oil, soybeans and soy products, omega-3 fortified eggs, and cod liver oil for two weeks before and during the study.
• Subjects will be required to avoid taking antioxidants (e.g., beta-carotene, selenium, vitamin C, vitamin E, zinc) for two weeks before and during the study.
• Subjects will be required to use olive oil exclusively for cooking, dressings, and sauces during the study and to avoid other vegetable oils because of their omega-3 contents.
• Because of reported cardioprotective effects of red wine, subjects will be required to avoid drinking red wine during the study.
• Subjects who are currently smoking or have smoking history within 1 year of study (defined as more than one pack of cigarettes in the past year) or have a greater than/equal to a 5 pack year smoking history.
• No exposure will be conducted within 2 weeks of a respiratory tract infection.
• Subject is pregnant, attempting to become pregnant or breastfeeding.
• Unspecified illnesses, which in the judgment of the investigators might increase the risk associated with PM inhalation will be a basis for exclusion.
• Have an allergy to latex.
• History of skin allergy to tape or electrodes.