Canola Oil, Fibre and DHA Enhanced Clinical Trial

The proposed study is a randomized, single-blind, crossover trial, it will be conducted at the Richardson Centre for Functional Food and Nutraceuticals (RCFFN), University of Manitoba. The study design will consist of 4 phases with 30 days per phase, each phase will be separated by 4-week washout periods. Participants will consume a recommended weight-maintaining diet (35% energy from fat, 50% carbohydrate, 15% protein) supplemented with the following novel Muffin and cookies: (a) control food containing butter, sunflower and safflower oil comprised largely of saturated fat with substantial levels of n-6 linoleic acid, and refined wheat flour common to current North American intakes, (b) food containing high oleic canola oil and docosahexaenoic acid (HOCO-DHA) and refined wheat flour, (c) food containing high molecular weight barley B-glucan and a combination of sunflower, safflower oil and butter, (d) food containing combination of HOCO-DHA and high molecular weight barley β-glucan. Treatments will be isocalorically incorporated into muffin and cookies consumed in equal parts at breakfast and supper.

Inclusion criteria

• BMI≥25 Kg/m2
• Waist circumference ≥94 cm (males) or ≥80 cm (females)

Meet at least two of the following:

• Triglycerides ≥1.7 mmol/L
• High density lipoprotein (HDL) cholesterol <1 mmol/L (males) or <1.3 mmol/L (females)
• Low density lipoprotein (LDL) cholesterol ≥2.7 mmol/L
• Fasting glucose ≥5.6 mmol/L

Exclusion criteria

• Consuming lipid lowering medications
• Consuming nutritional supplements
• Disease or disorder that could interfere with absorption
• Smokers
• Hypertension ≥150 mmHg (systolic) and/or ≥100 mmHg (diastolic)
• Planning to become pregnant
• Consume >1 alcoholic drink/day
• Medication within a month prior to screening