Cancer Genome Study Using Samples From Patients With Stage I or Stage II Non-Small Cell Lung Cancer Treated on Clinical Trial ACOSOG-Z0030

OBJECTIVES:

• To investigate the clinical relevance of conducting comprehensive molecular analyses on clinically annotated high-quality tumor biospecimens from patients with stage I or II, N0 or N1 (less than hilar) non-small cell lung cancer.

OUTLINE: This is a multicenter study.

Biological specimens are collected from participating clinical sites and analyzed by transcription profiling of RNA and microRNA; detection of DNA copy number changes and chromosomal rearrangements; epigenetic modifications analyses; and sequencing of genomic segments, genes, and regulatory regions to assess sequence variation. Clinical information associated with each specimen donor is also collected.

DISEASE CHARACTERISTICS:

• Diagnosis of non-small cell carcinoma

• Primary, untreated disease
• Stage I or II (T1 or T2, N0 or non-hilar N1, M0) disease
• Any of the following histologies allowed:

• Squamous cell carcinoma
• Adenocarcinoma (< 10%)
• Large cell carcinoma (high-grade)
• Available tumor tissue samples collected on clinical trial ACOSOG-Z0030 and meeting the following criteria:

• Snap-frozen and stored at a temperature of -80°C or lower
• Specimens may come from any site within the lung or one that is involved by direct regional extension of tumor from the originating site in the lung
• No regional lymph node or distant metastases
• No recurrent or persistent disease after prior neoadjuvant or adjuvant treatment for lung cancer
• Must have matching frozen samples of normal tissue and blood