BRAN Pilot Study: Metabolic Signature of Wheat Bran Related to Gut Fermentation in Humans

Inclusion criteria

• Subject able to understand the information given to him and having signed the informed consent form.
• subject having had a medical examination during the selection visit
• Healthy subject
• Female gender
• Age between 20 and 40 years (limits included)
• No Smoking
• Body mass index between 20 and 25 kg / m2 (limits included).
• fiber consumer ≥ 18 g / day
• Do not present any food allergy, nor food intolerance to the products of the study.
• Having no particular diet (vegetarian, vegan, high protein, etc.)
• Having a stool frequency ranging from 1 to 2 per day over the last 2 months
• Absence of pathology detectable by clinical examination and medical examination that may interfere with the evaluation criteria of the study.
• Sedentary subject or practicing steady regular physical activity during the entire study (maximum 4 hours per week).
• Accepting to submit to the protocol of the study with a day of hospitalization of 24h
• Stable weight in the last 3 months (+/- 5% of total body weight)
• Affiliation to a social security scheme.

Exclusion criteria

General criteria

• Subject presenting unstable medical or psychological conditions which, according to the investigator, could lead the subject to be non-compliant or uncooperative during the study or could compromise the safety or participation of the subject under study (according to Articles L.1121-6, L.1121-8, L.1121-9 and L1122-1-2 of the Public Health Code).
• Failure to respect the exclusion period of another study specified in the "National Volunteer File".
• Major subject under guardianship.
• Private subject of his liberty by judicial or administrative decision.
• Subject having exceeded the annual amount of compensation for participation in research protocols.
• Not having a refrigerator and / or freezer (necessary for the conservation of collections of urine and / or stool made at home) Biological criteria
• Total blood cholesterol> 11 mmol / L or blood triglycerides> 3 mmol / L
• Fasting blood glucose> 7 mmol / L
• CRP> 10 mg / L
• Demonstration of a biological abnormality judged by the investigator to be clinically significant
• Transaminases (ASAT and ALAT) and gamma GT greater than 1.5 times the upper limit of normal

Medical and therapeutic criteria:

• History of bariatric surgery
• History of digestive surgery with the exception of appendectomy
• Subject presenting gastrointestinal disorders such as ulcers, diverticula or inflammatory bowel diseases
• Diabetes type 1 or 2.
• Chronic renal failure.
• Chronic liver failure.
• known gastroparesis, gastrectomy, colectomy.
• History of chronic gastrointestinal disease with malabsorption.
• External pancreatic insufficiency.
• Known endocrine pathology that can interfere with carbohydrate metabolism (uncontrolled dysthyroidism, acromegaly, hypercorticism, ...).
• Subject with organic intestinal disease.
• subject who has undergone antibiotic therapy in the 3 months preceding the study may interfere with transit or intestinal microbiota:
• Subject treated with a drug that may interfere with carbohydrate metabolism:
• Corticosteroids.
• anorectics.
• High dose gastric bandages.
• Subject having a concomitant pathology incompatible with the constraints of the protocol or which may interfere with the evaluation of the main criterion.
• PA systolic not between 100 and 140 mmHg and diastolic not between 50 and 90 mmHg
• Dieting or wishing to lose weight
• Pregnant woman or wishing to be pregnant or breastfeeding (interrogation data)
• Alcoholism or abuse or dependence on another proven drug. Consumption of more than 3 alcoholic beverages per day is considered abusive. An alcoholic beverage is 30 mL of spirits, 120 mL of wine or 330 mL of beer
• Donation of blood in the 2 months preceding the selection visit
• Subjects consuming dietary supplements
• Subjects refusing to follow the dietary instructions on the 3 days preceding the test day